MAdCAM-1 Rat anti-Mouse, Brilliant Violet 421, Clone: MECA-367, BD Optibuild™

Catalog No.	Quantity
BDB742812	50 μg

Catalog No. BDB742812 Supplier BD Biosciences Supplier No. 742812

Description

Rat Monoclonal Antibody

The MECA-367 monoclonal antibody specifically recognizes mucosal vascular addressin MAdCAM-1. In the fetus and neonate, MAdCAM-1 is the predominant vascular addressin on the high endothelial venules (HEV) of peripheral lymph nodes. In adult mice, MAdCAM-1 is preferentially expressed in mucosal lymphoid tissues and lamina propria; it is also expressed on sinus-lining cells in the spleen. MAdCAM-1 expression is upregulated on the HEV of peripheral lymph nodes in adult NOD mice4 and is involved in the development of diabetes and insulitis. Furthermore, there is evidence that IFN-γcan induce MAdCAM-1 expression in non-mucosal sites in adult mice. MAdCAM-1 is a predominant ligand for integrinα4β7, a lymphocyte mucosal homing receptor, and a facultative ligand for CD62L (L-selectin). MECA-367 mAb binds to the first domain of MAdCAM-1 and blocks MAdCAM-1-dependent binding in vitro /and lymphocyte homing to Peyer's patch HEV in vivo /. Source of the immunogen was endothelial cells from BALB/c mouse mesenteric and peripheral lymph nodes.

The antibody was conjugated to BD Horizon™ BV421 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 407nm and Em Max at 421nm, BD Horizon BV421 can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50nm filter). BD Horizon BV421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue conjugates. BV421 is a polymer-based dye excited by the violet laser and is one of the brightest fluorochromes offered by BD Biosciences. Conjugates are typically 10 times brighter than Pacific Blue™ conjugates and are often as bright as or brighter than PE conjugates. Due to nearly identical excitation and emission properties but different spillover characteristics, BD Horizon BV421, Pacific Blue™, and BD Horizon™ V450 cannot be used simultaneously.

Specifications

• Antigen: MAdCAM-1
• Classification: Monoclonal
• Clone: MECA-367
• Concentration: 0.2mg/mL
• Conjugate: Brilliant Violet 421
• Formulation: Aqueous buffered solution containing ≤0.09% sodium azide.
• Gene Alias: Madcam1, MADCA, mucosal vascular addressin cell adhesion molecule 1
• Host Species: Rat
• Immunogen: Mouse endothelial cells from BALB/c mouse mesenteric and peripheral lymph nodes.
• Purification Method: Affinity Purified
• Quantity: 50 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Target Species: Mouse
• Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
• Isotype: IgG2a κ

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov