MAdCAM-1 Rat anti-Mouse, BUV395, Clone: MECA-89, BD Optibuild™

Catalog No.	Quantity
BDB744112	50 μg

Catalog No. BDB744112 Supplier BD Biosciences Supplier No. 744112

Description

Rat Monoclonal Antibody

The MECA-89 antibody specifically recognizes the mucosal vascular addressin cell adhesion molecule (MAdCAM-1). In the fetus and neonate, MAdCAM-1 is the predominant vascular addressin on the high endothelial venules (HEV) of peripheral lymph nodes. In adult mice, MAdCAM-1 is preferentially expressed in mucosal lymphoid tissues and lamina propria; it is also expressed on sinus-lining cells in the spleen. MAdCAM-1 expression is upregulated on the HEV of peripheral lymph nodes in adult NOD mice and is involved in the development of diabetes and insulitis. Furthermore, there is evidence that IFN-γcan induce MAdCAM-1 expression in non-mucosal sites in adult mice. MAdCAM-1 is a predominant ligand for integrinα4β7, a lymphocyte mucosal homing receptor, and a facultative ligand for CD62L (L-selectin). MECA-89 mAb binds to the second domain of MAdCAM-1 and does not block MAdCAM-1-dependent binding in vitro /. Source of the immunogen was endothelial cells from BALB/c mouse mesenteric and peripheral lymph nodes.

The antibody was conjugated to BD Horizon™ BUV395 which is part of the BD Horizon Brilliant™ Ultraviolet family of dyes. This dye has been exclusively developed by BD Biosciences to have minimal spillover into other detectors, making it an optimal choice for multicolor flow cytometry. With an Ex Max at 348 nm and an Em Max at 395 nm, BD Horizon BUV395 can be excited with a 355 nm laser and detected with a 379/28 filter. BUV395 is a UV-excitable dye that has been developed exclusively by BD Biosciences. With an excitation max of 348 nm and emission max of 395 nm, BUV395 can be excited by the 355nm laser and detected in a 379/28 filter. This dye is optimal for multicolor flow cytometry because it has little to no spillover into other detectors.

Specifications

• Antigen: MAdCAM-1
• Classification: Monoclonal
• Clone: MECA-89
• Concentration: 0.2mg/mL
• Conjugate: Brilliant Ultraviolet 395
• Formulation: Aqueous buffered solution containing ≤0.09% sodium azide.
• Gene Alias: MAdCAM-1, Madcam1, mMAdCAM-1, MADCA
• Host Species: Rat
• Immunogen: Mouse mesenteric and peripheral lymph node cells
• Purification Method: Affinity Purified
• Quantity: 50 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Target Species: Mouse
• Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
• Isotype: IgG2a κ

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov