Invitrogen™ MANEA Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA532000

Catalog No. PIPA532000

Description

Recommended positive controls: A431, HeLa, HepG2. Predicted reactivity: Mouse (81%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

N-glycosylation of proteins is initiated in the endoplasmic reticulum (ER) by the transfer of the preassembled oligosaccharide glucose-3-mannose-9-N-acetylglucosamine-2 from dolichyl pyrophosphate to acceptor sites on the target protein by an oligosaccharyltransferase complex. This core oligosaccharide is sequentially processed by several ER glycosidases and by an endomannosidase (E.C. 3.2.1.130), such as MANEA, in the Golgi. MANEA catalyzes the release of mono-, di-, and triglucosylmannose oligosaccharides by cleaving the alpha-1,2-mannosidic bond that links them to high-mannose glycans (Hamilton et al., 2005).

Specifications

• Antigen: MANEA
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: MANEA
• Gene Alias: 4932703L02Rik; alpha 1,2-endomannosidase; ENDO; Endo-alpha mannosidase; endomannosidase; glycoprotein endo-alpha-1,2-mannosidase; hEndo; Mandaselin; Manea; mannosidase endo-alpha; mannosidase, endo-alpha; mEndo
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 242362, 79694
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Immunohistochemistry (Paraffin), Western Blot
• Concentration: 1 mg/mL
• Formulation: 0.1M tris glycine with 20% glycerol and 0.01% thimerosal; pH 7
• Gene Accession No.: Q5SRI9, Q6NXH2
• Gene Symbols: MANEA
• Immunogen: Recombinant protein encompassing a sequence within the center region of human MANEA. The exact sequence is proprietary.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse
• Product Type: Antibody
• Isotype: IgG

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov