Invitrogen™ MANEA Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA588029

Catalog No. PIPA588029

Description

Immunogen sequence: RPNTATFGAP FGLDLLPELH QRTIHLGKNF DFQKSDRINS ETNTKNLKSV EITMKPSKAS ELNLDELPPL N; Positive Samples: 293T, U-87MG; Cellular Location: Golgi apparatus membrane, Single-pass type II membrane protein.

N-glycosylation of proteins is initiated in the endoplasmic reticulum (ER) by the transfer of the preassembled oligosaccharide glucose-3-mannose-9-N-acetylglucosamine-2 from dolichyl pyrophosphate to acceptor sites on the target protein by an oligosaccharyltransferase complex. This core oligosaccharide is sequentially processed by several ER glycosidases and by an endomannosidase (E.C. 3.2.1.130), such as MANEA, in the Golgi. MANEA catalyzes the release of mono-, di-, and triglucosylmannose oligosaccharides by cleaving the alpha-1,2-mannosidic bond that links them to high-mannose glycans (Hamilton et al., 2005).

Specifications

• Antigen: MANEA
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: MANEA
• Gene Alias: 4932703L02Rik; alpha 1,2-endomannosidase; ENDO; Endo-alpha mannosidase; endomannosidase; glycoprotein endo-alpha-1,2-mannosidase; hEndo; Mandaselin; Manea; mannosidase endo-alpha; mannosidase, endo-alpha; mEndo
• Host Species: Rabbit
• Purification Method: Affinity Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 79694
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid

• Applications: ELISA, Western Blot
• Concentration: 0.33 mg/mL
• Formulation: PBS with 50% glycerol and 0.01% thimerosal; pH 7.3
• Gene Accession No.: Q5SRI9
• Gene Symbols: MANEA
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 30-100 of human MANEA (NP_0789172).
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG