Promotional price valid on web orders only. Your contract pricing may differ. Interested in signing up for a dedicated account number?
Learn More

Marginal Zone B Cells Mouse Anti-Rat, BUV395, Clone: HIS57, BD Optibuild™

Mouse Monoclonal Antibody

Supplier:  BD Biosciences 743347

Catalog No. BDB743347


Only null left
Add to Cart

Description

Description

The HIS57 monoclonal antibody reacts with an unknown antigen that is highly expressed by most marginal zone B (MZ-B) cells in the spleen. In contrast, this antigen is weakly expressed, or not expressed at all, by other B-cell subpopulations. Rat MZ-B cells express low levels of CD45R (mAb HIS24) and sIgD and high levels of sIgM. The HIS57 mAb does not stain granulocytes and thymocytes. Immunohistochemical staining of normal spleen sections with HIS57 mAb produced a positive signal in the marginal zone and, to a lesser extent, in B-cell follicles. This marker can be used in combination with CD45R, sIgD, and sIgM t0 identify MZ-B cells in the rat.

The antibody was conjugated to BD Horizon™ BUV395 which is part of the BD Horizon Brilliant™ Ultraviolet family of dyes. This dye has been exclusively developed by BD Biosciences to have minimal spillover into other detectors, making it an optimal choice for multicolor flow cytometry. With an Ex Max at 348 nm and an Em Max at 395 nm, BD Horizon BUV395 can be excited with a 355 nm laser and detected with a 379/28 filter. BUV395 is a UV-excitable dye that has been developed exclusively by BD Biosciences. With an excitation max of 348 nm and emission max of 395 nm, BUV395 can be excited by the 355nm laser and detected in a 379/28 filter. This dye is optimal for multicolor flow cytometry because it has little to no spillover into other detectors.
TRUSTED_SUSTAINABILITY
Specifications

Specifications

Marginal Zone B Cells
HIS57
Brilliant Ultraviolet 395
Mouse
Affinity Purified
RUO
Mouse
IgG1 κ
Monoclonal
0.2mg/mL
Aqueous buffered solution containing ≤0.09% sodium azide.
Rat Splenic B Cells
50 μg
Primary
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Product Suggestions

Product Suggestions

Videos
Safety and Handling

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov
SDS
Documents

Documents

Product Certifications
Promotions

Promotions

Product Content Correction

Your input is important to us. Please complete this form to provide feedback related to the content on this product.

Product Title

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.

Your feedback has been submitted: Thank you for helping us improve our website.