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Marginal Zone B Cells Mouse Anti-Rat, BUV395, Clone: HIS57, BD Optibuild™
Mouse Monoclonal Antibody
Supplier: BD Biosciences 743347
Description
The HIS57 monoclonal antibody reacts with an unknown antigen that is highly expressed by most marginal zone B (MZ-B) cells in the spleen. In contrast, this antigen is weakly expressed, or not expressed at all, by other B-cell subpopulations. Rat MZ-B cells express low levels of CD45R (mAb HIS24) and sIgD and high levels of sIgM. The HIS57 mAb does not stain granulocytes and thymocytes. Immunohistochemical staining of normal spleen sections with HIS57 mAb produced a positive signal in the marginal zone and, to a lesser extent, in B-cell follicles. This marker can be used in combination with CD45R, sIgD, and sIgM t0 identify MZ-B cells in the rat.
The antibody was conjugated to BD Horizon™ BUV395 which is part of the BD Horizon Brilliant™ Ultraviolet family of dyes. This dye has been exclusively developed by BD Biosciences to have minimal spillover into other detectors, making it an optimal choice for multicolor flow cytometry. With an Ex Max at 348 nm and an Em Max at 395 nm, BD Horizon BUV395 can be excited with a 355 nm laser and detected with a 379/28 filter. BUV395 is a UV-excitable dye that has been developed exclusively by BD Biosciences. With an excitation max of 348 nm and emission max of 395 nm, BUV395 can be excited by the 355nm laser and detected in a 379/28 filter. This dye is optimal for multicolor flow cytometry because it has little to no spillover into other detectors.
Specifications
Marginal Zone B Cells | |
HIS57 | |
Brilliant Ultraviolet 395 | |
Mouse | |
Affinity Purified | |
RUO | |
Mouse | |
IgG1 κ |
Monoclonal | |
0.2mg/mL | |
Aqueous buffered solution containing ≤0.09% sodium azide. | |
Rat Splenic B Cells | |
50 μg | |
Primary | |
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. |
Safety and Handling
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