Invitrogen™ Maspin Polyclonal Antibody, Biotin, PeproTech®, Invitrogen™

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ 500P270BT500UG

Catalog No. 50-271-8549

Description

AA Sequence of recombinant protein: MDALQLANSA FAVDLFKQLC EKEPLGNVLF SPICLSTSLS LAQVGAKGDT ANEIGQVLHF ENVKDIPFGF QTVTSDVNKL SSFYSLKLIK RLYVDKSLNL STEFISSTKR PYAKELETVD FKDKLEETKG QINNSIKDLT DGHFENILAD NSVNDQTKIL VVNAAYFVGK WMKKFPESET KECPFRLNKT DTKPVQMMNM EATFCMGNID SINCKIIELP FQNKHLSMFI LLPKDVEDES TGLEKIEKQL NSESLSQWTN PSTMANAKVK LSIPKFKVEK MIDPKACLEN LGLKHIFSED TSDFSGMSET KGVALSNVIH KVCLEITEDG GDSIEVPGAR ILQHKDELNA DHPFIYIIRH NKTRNIIFFG KFCSP. Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human Maspin. Anti-Human Maspin-specific antibody was purified by affinity chromatography and then biotinylated. Sandwich ELISA: To detect hMaspin by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human Maspin (500-P270) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant hMaspin. Western Blot: To detect hMaspin by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hMaspin is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. 500-P270BT-1MG will be provided as 2 x 500 μg

SERPINB5 is a tumor suppressor. It blocks the growth, invasion, and metastatic properties of mammary tumors. As it does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, it exhibits no serine protease inhibitory activity.

Specifications

• Antigen: Maspin
• Classification: Polyclonal
• Conjugate: Biotin
• Gene: SERPINB5
• Gene Alias: 1110036M19Rik; AI462524; AI646751; Maspin; ovalbumin; peptidase inhibitor 5; PI5; PI-5; protease inhibitor 5; protease inhibitor 5 (maspin); serine (or cysteine) peptidase inhibitor, clade B, member 5; serine (or cysteine) proteinase inhibitor, clade B (ovalbumin), member 5; serine (or cysteine) proteinase inhibitor, clade B, member 5; serine protease inhibitor 7; serpin B5; serpin family B member 5; serpin peptidase inhibitor, clade B (ovalbumin), member 5; SERPINB5; Spi5; Spi7
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 5268
• Content And Storage: -20°C
• Form: Lyophilized

• Applications: ELISA, Western Blot
• Concentration: 0.1-1.0 mg/mL
• Formulation: PBS with no preservative
• Gene Accession No.: P36952
• Gene Symbols: SERPINB5
• Immunogen: E.coli-derived Recombinant Human Maspin.
• Quantity: 500 μg
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG