MASTL Rabbit anti-Human, Polyclonal, Invitrogen
Rabbit Polyclonal Antibody
Manufacturer: Invitrogen PA550180
The antibody detects endogenous levels of total MASTL protein.Serine/threonine kinase that plays a key role in M phase by acting as a regulator of mitosis entry and maintece. Acts by promoting the inactivation of protein phosphatase 2A (PP2A) during M phase: does not directly inhibit PP2A but acts by mediating phosphorylation and subsequent activation of ARPP19 and ENSA at ft.Ser-62 ft. and ft.Ser-67 ft., respectively. ARPP19 and ENSA are phosphatase inhibitors that specifically inhibit the PPP2R2D (PR55-delta) subunit of PP2A. Inactivation of PP2A during M phase is essential to keep cyclin-B1-CDK1 activity high. Following DNA damage, it is also involved in checkpoint recovery by being inhibited. Phosphorylates histone protein in vitro; however such activity is unsure in vivo. May be involved in megakaryocyte differentiation. Ota T., Nat. Genet. 36:40-45(2004). Deloukas P., Nature 429:375-381(2004). Beausoleil S.A., Nat. Biotechnol. 24:1285-1292(2006).
|Synthesized peptide derived from C-terminal of human MASTL.|
|Antigen affinity chromatography|
|PBS with 50% glycerol and 0.02% sodium azide; pH 7.4|
|GW, GWL, THC2|