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  6. Invitrogen™ Mature Macrophage Marker Monoclonal Antibody (eBio25F9 (25F9)), eBioscience™, Invitrogen™ 

Invitrogen™ Mature Macrophage Marker Monoclonal Antibody (eBio25F9 (25F9)), eBioscience™, Invitrogen™
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Catalog No. 5012422
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100 μg
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Catalog No. Quantity
50-124-22 100 μg
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Catalog No. 50-124-22 Supplier Invitrogen™ Supplier No. 14011582
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Description

Mouse Monoclonal Antibody

Description: The monoclonal antibody 25F9 recognizes a protein on mature macrophages both on the cell surface and in intracellular vesicular structures. Expression is thought to correlate with endocytic macrophages and is useful in many disease models. Expression is not found on immature macrophages or monocytes or any other hematopoietic cell. Applications Reported: This eBio25F9 (25F9) antibody has been reported for use in flow cytometric analysis, immunoprecipitation, immunoblotting (WB), immunohistology staining of frozen and paraffin embedded tissue sections. Applications Tested: This eBio25F9 (25F9) antibody has been tested by flow cytometric analysis of human macrophages. This can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 μm post-manufacturing filtered.

Mature Macrophage Marker Monoclonal antibody specifically detects Mature Macrophage Marker in Human samples. It is validated for Flow Cytometry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot.
TRUSTED_SUSTAINABILITY

Specifications

Antigen Mature Macrophage Marker
Applications Flow Cytometry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot
Classification Monoclonal
Clone eBio25F9 (25F9)
Concentration 0.5 mg/mL
Conjugate Unconjugated
Formulation PBS with 0.09% sodium azide; pH 7.2
Gene Alias ED2-like antigen; Macrophages; Mature Macrophages
Host Species Mouse
Purification Method Affinity chromatography
Quantity 100 μg
Regulatory Status RUO
Primary or Secondary Primary
Target Species Human
Content And Storage 4°C
Product Type Antibody
Form Liquid
Isotype IgG1 κ
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For Research Use Only.

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