Invitrogen™ MAVS Monoclonal Antibody (ABM28H9), PE, eBioscience™, Invitrogen™

Catalog No.	Quantity
12-983-542	100 Tests

Catalog No. 12-983-542 Supplier Invitrogen™ Supplier No. 12983542

Description

Mouse Monoclonal Antibody

Description: This ABM28H9 monoclonal antibody recognizes human MAVS, a mitochondrial membrane protein also known as VISA, CARDIF or IPS-1. Applications Reported: This ABM18H9 antibody has been reported for use in intracellular staining followed by flow cytometric analysis. Applications Tested: This ABM18H9 antibody has been pre-diluted and tested by intracellular staining followed by flow cytometric analysis of HEL cells using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) and protocol. Please refer to Staining Intracellular Antigens for Flow Cytometry, Protocol A: Two step protocol for intracellular (cytoplasmic) proteins located at www.thermofisher.com/flowprotocols. This may be used at 5 μL (0.06 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. eFluor 450 is an alternative to Pacific Blue. eFluor 450 emits at 446 nm and is excited with the violet laser line (405 nm). Please make sure that your instrument is capable of detecting this fluorochrome. Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser

Two distinct signaling pathways activate the host innate immunity against viral infection. One pathway is reliant on members of the Toll-like receptor (TLR) family while the other uses the RNA helicase RIG-I as a receptor for intracellular viral double-stranded RNA as a trigger for the immune response. MAVS is a mitochondrial membrane protein that was identified as a critical component in the IFN beta signaling pathways that recruits IRF-3 to RIG-I, leading to its activation and that of NF-kappa-B. MAVS is also thought to interact with other components of the innate immune pathway such as the TLR adapter protein TRIF, TRAF2 and TRAF6. MAVS also interacts with the IKK-alpha, IKK-beta and IKK-iota kinases through its C-terminal region. Cleavage of this region by the Hepatitis C virus (HCV) protease allows HCV to escape the host immune system. Multiple isoforms of MAVS are known to exist.

Specifications

• Antigen: MAVS
• Applications: Flow Cytometry
• Classification: Monoclonal
• Clone: ABM28H9
• Concentration: 5 μL/Test
• Conjugate: PE
• Formulation: PBS with BSA and 0.09% sodium azide; pH 7.2
• Gene: MAVS
• Gene Accession No.: Q7Z434
• Gene Alias: CARD adapter inducing interferon beta; CARD adaptor inducing IFN-beta; Cardif; D430028G21Rik; IFN-B promoter stimulator 1; IFN-beta promoter stimulator-1; interferon beta promoter stimulator protein 1; interferon-beta promoter stimulator protein 1; IPS1; IPS-1; KIAA1271; MAVS; mitochondrial antiviral signaling protein; mitochondrial anti-viral signaling protein; mitochondrial antiviral-signaling protein; Putative NF-kappa-B-activating protein 031N; virus-induced signaling adapter; virus-induced signaling adaptor; virus-induced-signaling adapter; VISA
• Gene Symbols: MAVS
• Host Species: Mouse
• Immunogen: Recombinant fragment of human MAVS (aa 72-296).
• Purification Method: Affinity chromatography
• Quantity: 100 Tests
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 57506
• Target Species: Human
• Content And Storage: 4°C, store in dark, DO NOT FREEZE!
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG2b κ

Safety and Handling

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