MBD2 Polyclonal Antibody, Active Motif™

Rabbit Polyclonal Antibody

Supplier:  Active Motif 39548

Catalog No. 50-199-2714

Description

Heterochromatin protein-1 (HP1) is a methyl-lysine binding protein localized at heterochromatin sites, where it mediates gene silencing. It has been shown that mammalian methyltransferases that selectively methylate histone H3 on lysine-9 generate a binding site for HP1 proteins, a family of heterochromatic adaptor molecules implicated in both gene silencing and supranucleosomal chromatin structure. High-affinity in vitro recognition of a methylated histone H3 peptide by HP1 requires a functional chromodomain. Thus, the HP1 chromodomain is a specific interaction motif for the methyl epitope on lysine-9 of histone H3. In vivo, heterochromatin association of HP1 proteins is lost in Suv39h double-null primary mouse fibroblasts but is restored after reintroduction of a catalytically active SUV39H1 HMTase.

Specifications

• Antigen: MBD2
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: Mbd2
• Gene Alias: Demethylase; DKFZp586O0821; DMTase; MBD2; MBD2a; methyl-CpG binding domain protein 2; Methyl-CpG-binding domain protein 2; Methyl-CpG-binding protein MBD2; NY-CO-41
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 8932
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid

• Applications: Western Blot
• Concentration: Lot-Specific
• Formulation: 70mM tris with 0.07mM EDTA, 105mM NaCl, 30% glycerol, 31mM glycine and 0.035% sodium azide; pH 8
• Gene Accession No.: Q9UBB5
• Gene Symbols: Mbd2
• Immunogen: This MBD2 antibody was raised against a synthetic peptide corresponding to human MBD2.
• Quantity: 10 μL
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG