Invitrogen™ MBD4 Polyclonal Antibody

Catalog No.	Quantity
PIPA578272	100 μL

Catalog No. PIPA578272 Supplier Invitrogen™ Supplier No. PA578272

Description

Rabbit Polyclonal Antibody

Positive Control: 3xFlag-human MBD4-transfected 293T Predicted Reactivity: Chimpanzee (97%) Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

DNA methylation, or the addition of methyl groups to cytosine bases in the dinucleotide CpG, is imperative to proper development and regulates gene expression. The methylation pattern involves the enzymatic processes of methylation and demethylation. The demethylation enzyme was recently found to be a mammalian protein, which exhibits demethylase activity associated to a methyl-CpG-binding domain (MBD). The enzyme is able to revert methylated cytosine bases to cytosines within the particular dinucleotide sequence mdCpdG by catalyzing the cleaving of the methyl group as methanol. MeCP2 and MBD1 (PCM1) are first found to repress transcription by binding specifically to methylated DNA. MBD2 and MBD4 (also known as MED1) were later found to colocalize with foci of heavily methylated satellite DNA and believed to mediate the biological functions of the methylation signal. Surprisingly, MBD3 does not bind methylated DNA both in vivo and in vitro. MBD1, MBD2, MBD3, and MBD4 are found to be expressed in somatic tissues, but the expression of MBD1 and MBD2 is reduced or absent in embryonic stem cells, which are known to be deficient in MeCP1 activity. MBD4 have homology to bacterial base excision repair DNA N-glycosylases/lyases. In some microsatellite unstable tumors MBD4 is mutated at an exonic polynucleotide tract.

Specifications

• Antigen: MBD4
• Applications: Western Blot, Immunocytochemistry
• Classification: Polyclonal
• Concentration: 1 mg/mL
• Conjugate: Unconjugated
• Formulation: PBS with 20% glycerol and 0.025% ProClin 300; pH 7
• Gene: MBD4
• Gene Accession No.: O95243
• Gene Alias: 3,N(4)-ethenocytosine glycosylase; G/5-fluorouracil mismatch glycosylase with biphasic kinetics; G/T mismatch glycosylase; G/U mismatch glycosylase; Mbd4; MED1; methyl-CpG binding domain 4 DNA glycosylase; methyl-CpG binding domain 4, DNA glycosylase; methyl-CpG binding domain protein 4; methyl-CpG-binding domain protein 4; Methyl-CpG-binding endonuclease 1; methyl-CpG-binding protein MBD4; Mismatch-specific DNA N-glycosylase; putative methyl-CpG binding protein
• Gene Symbols: MBD4
• Host Species: Rabbit
• Immunogen: Recombinant protein encompassing a sequence within the center region of human MBD4. The exact sequence is proprietary.
• Purification Method: Antigen Affinity Chromatography
• Quantity: 100 μL
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 8930
• Target Species: Human
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov