Thermo Scientific™ McCoy's 5A Media for SILAC PROMO

Description

Analyze protein expression by mass spectrometry using these media or additives for stable isotope labeling with amino acids in cell culture (SILAC).

SILAC is a powerful method to identify and quantify relative differential changes in complex protein samples. This approach entails the in vivo metabolic incorporation of heavy ¹³C- or ¹⁵N-labeled amino acids into proteins followed by mass spectrometry (MS) for the accelerated and comprehensive identification, characterization and quantitation of proteins.

Highlights:

• Flexible–liquid media deficient in both L-lysine and L-arginine and powdered media deficient in L-leucine, L-lysine and L-arginine, allowing for more complete proteome coverage through multiple isotopic amino acid labeling

• Compatible–DMEM, MEM, RPMI-1640, IMDM, Ham's F12 and McCoy's 5A media and dialyzed FBS are used to culture a variety of cell lines, including HeLa, 293T, COS7, U2OS, A549, A431, HepG2, NIH 3T3, K562 and Jurkat

• Optimized, specialized media–the Expansion DMEM and Low-Osmolarity DMEM are specifically designed and qualified for mouse embryonic stem cell propagation and SILAC protein expression profiling with mass spectrometry

• High quality–Stem Cell Screened Dialyzed FBS is tested to ensure that it does not promote stem cell differentiation

Specifications

• Formulation: McCoy's 5A media minus L-Lysine and L-Arginine
• For Use With (Equipment): Mass Spectrometer
• Product Type: Media
• Content And Storage: Store in refrigerator (2°C to 8°C).
• Shipping Condition: Wet Ice
• Starting Material: Cells
• Final Product Type: Protein
• Workflow Step: Protein Labeling
• Quantity: 500 mL
• Form: Liquid

For Research Use Only. Not for use in diagnostic procedures.