mCherry Recombinant Rat Monoclonal Antibody (16D7), Alexa Fluor™ Plus 555, Invitrogen™

Rat Recombinant Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
74-000-3TP555	Invitrogen™ 740003TP555	50 μL
50-274-0588	Invitrogen™ 740003TP55520UG	20 μL

Description

mCherry Tag recombinant rat monoclonal antibody specifically recognizes mCherry Tag at both C and N-terminus of proteins. Alexa Fluor™ Plus recombinant antibodies are conjugated using new, proprietary dye chemistry so you can generate stunning data. Alexa Fluor™ Plus antibodies represent an advancement in fluorescent conjugate technology. Alexa Fluor™ Plus antibodies provide brighter signal compared to leading Alexa Fluor™ antibodies, providing you with better signal-to-noise for your critical experiments. These antibodies show better specificity and lot-to-lot consistency as these are recombinant antibodies, generated by cloning specific genes for the desired antibodies into an expression vector and expressed in vitro. Centrifuge the protein conjugate solution briefly in a microcentrifuge before use; add only the supernatant to the experiment. This step will help eliminate any protein aggregates that may have formed during storage, thereby reducing nonspecific background staining. This 16D7 antibody has been tested by WB and FC analysis using HEK-293E cells that were transiently transfected with mCherry-protein constructs. This may be used for WB at 1ug/mL and for FC at 0.5ug per test. Excitation: 553 nm; Emission: 568 nm; Laser: Yellow Laser Filtration: 0.2um post-manufacturing filtered. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

mCherry Recombinant Monoclonal antibody specifically detects mCherry in Tag samples. It is validated for Flow Cytometry, Western Blot

Specifications

• Antigen: mCherry
• Concentration: 1.0 mg/mL
• Classification: Recombinant Monoclonal
• Form: Liquid
• Regulatory Status: RUO
• Formulation: proprietary buffer with 0.008% Bromonitrodioxane, 0.008% Methylisothiazolone; pH 6.8
• Immunogen: Full-length protein mCherry.
• Primary or Secondary: Primary
• Content And Storage: 4°C, store in dark, DO NOT FREEZE!

• Clone: 16D7
• Applications: Flow Cytometry, Western Blot
• Conjugate: Alexa Fluor Plus 555
• Host Species: Rat
• Target Species: Chemical
• Gene Alias: mCherry
• Isotype: IgG2a κ
• Purification Method: Affinity chromatography
• Product Type: Antibody