Invitrogen™ MCM2 Monoclonal Antibody (SP85)

Rabbit Monoclonal Antibody

Supplier:  Invitrogen™ MA516377

Catalog No. PIMA516377

Description

Heat-mediated antigen retrieval is recommended prior to staining, using a 1mM EDTA buffer, pH 8.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 30 min at room temperature. A suggested positive control is rectal carcinoma.

MCM2 (Mini chromosome maintenance protein 2) is involved in regulating DNA replication. MCM2 (also called CDCL1, mitotin and BM28), is a human nuclear protein that is crucial in the cell cycle, being involved in the onset of DNA replication and cell division. It is similar to members of the family of early S-phase proteins. MCM2 has been mapped to 3q21. From its localization, CDCL1 became a candidate for an oncogene affected by chromosomal breaks in acute myeloid leukemia (AML).

Specifications

• Antigen: MCM2
• Classification: Monoclonal
• Conjugate: Unconjugated
• Gene: MCM2
• Gene Alias: AA959861; AW476101; BM28; CCNL1; cdc19; CDCL1; cell devision cycle-like 1; cyclin-like 1; D3S3194; DFNA70; DNA replication licensing factor MCM2; Kiaa0030; MCM DNA helicase complex subunit MCM2; Mcm2; Mcmd2; MGC10606; mini chromosome maintenance deficient 2; minichromosome maintenance complex component 2; minichromosome maintenance deficient 2 (mitotin); minichromosome maintenance deficient 2 mitotin; minichromosome maintenance deficient 2 mitotin (S. cerevisiae); Minichromosome maintenance protein 2; Minichromosome maintenance protein 2 homolog; MITOTIN; mKIAA0030; Nuclear protein BM28; YBL023C; YBL0438
• Host Species: Rabbit
• Purification Method: Protein A/G
• Regulatory Status: RUO
• Gene ID (Entrez): 17216, 312538, 4171
• Content And Storage: 4°C
• Form: Liquid

• Applications: Flow Cytometry, Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry
• Clone: SP85
• Formulation: PBS with 1% BSA and 0.1% sodium azide; pH 7.6
• Gene Accession No.: P49736, P97310
• Gene Symbols: MCM2
• Immunogen: Synthetic peptide derived from internal region of human MCM2 protein.
• Quantity: 500 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG