Invitrogen™ MCM3 Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA529106

Catalog No. PIPA529106

Description

Recommended positive controls: 293T, A431, H1299, HeLa, HepG2, Molt-4, Raji. Predicted reactivity: Mouse (95%), Rat (94%), Zebrafish (80%), Xenopus laevis (84%), Chicken (86%), Rhesus Monkey (99%), Bovine (97%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein is a subunit of the protein complex that consists of MCM2-7. It has been shown to interact directly with MCM5/CDC46. This protein also interacts with, and thus is acetlyated by MCM3AP, a chromatin-associated acetyltransferase. The acetylation of this protein inhibits the initiation of DNA replication and cell cycle progression.

Specifications

• Antigen: MCM3
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: MCM3
• Gene Alias: AL033361; C80350; cervical cancer proto-oncogene 5; DNA polymerase alpha holoenzyme-associated protein P1; DNA replication factor MCM3; DNA replication licensing factor MCM3; HCC5; hRlf beta subunit; MCM3; MCM3 minichromosome maintenance deficient 3; Mcmd; Mcmd3; MGC1157; mini chromosome maintenance deficient; minichromosome maintenance complex component 3; minichromosome maintenance deficient 3; minichromosome maintenance deficient 3 (S. cerevisiae); P1; P1.h; p1.m; p102; P1-MCM3; replication licensing factor, beta subunit; RLF subunit beta; RLFB; RP1-108C2.3
• Host Species: Rabbit
• Purification Method: Antigen Affinity Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 17215, 4172
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: ChIP Assay, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot, Immunocytochemistry
• Concentration: 0.71 mg/mL
• Formulation: 0.1M tris glycine with 10% glycerol and 0.01% thimerosal; pH 7
• Gene Accession No.: P25205, P25206
• Gene Symbols: MCM3
• Immunogen: Recombinant protein fragment corresponding to a region within amino acids 418 and 657 of Human MCM3.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse
• Product Type: Antibody
• Isotype: IgG