Invitrogen™ MCM7 Monoclonal Antibody (3H11)

Catalog No.	Quantity
PIMA549205	100 μg

Catalog No. PIMA549205 Supplier Invitrogen™ Supplier No. MA549205

Description

Mouse Monoclonal Antibody

Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL. Human MCM7 shares 94% amino acid (aa) sequence identity with MCM7. Positive Control - WB: human Hela whole cell, human MCF-7 whole cell, human MDA-MB-231 whole cell, rat C6 whole cell, human Hela whole cell, human K562 whole cell, human Jurkat whole cell, human MCF-7 whole cell, human 293T whole cell, human U2OS whole cell, human A549 whole cell. IHC: human laryngeal squamous cell carcinoma tissue, human adenocarcinoma of the colon tissue, human chronic tonsillitis tissue. ICC/IF: PC-3 cell. Flow: MCF-7 cell. Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

MCM7 encodes a protein that is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by the MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 4 and 6 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme.The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by the MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 4 and 6 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. Cyclin D1-dependent kinase, CDK4, is found to associate with this protein, and may regulate the binding of this protein with the tumorsuppressor protein RB1/RB. Alternatively spliced transcript variants encoding distinct isoforms have been reported.

Specifications

• Antigen: MCM7
• Applications: Flow Cytometry, Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry
• Classification: Monoclonal
• Clone: 3H11
• Concentration: 500 μg/mL
• Conjugate: Unconjugated
• Formulation: PBS with 4mg trehalose and no preservative
• Gene: Mcm7
• Gene Accession No.: P33993
• Gene Alias: AI747533; CDC47; CDC47 homolog; CDC47 homolog A; CDC47p; DNA replication licensing factor MCM7; DNA replication licensing factor mcm7-A; DNA replication licensing factor MCM7-like protein; homolog of S. cerevisiae Cdc47; mCDC47; MCM2; MCM7; MCM7 minichromosome maintenance deficient 7; mcm7.S; mcm7-a; mcm7-b; Mcmd7; mini chromosome maintenance deficient 7; minichromosome maintenance complex component 7; minichromosome maintenance complex component 7 S homeolog; minichromosome maintenance deficient 7; minichromosome maintenance deficient 7 (S. cerevisiae); minichromosome maintenance protein 7; minichromosome maintenance protein 7-A; P1 P85MCM; P1.1-MCM3; P1CDC47; P85MCM; p90; PNAS146; PPP1R104; protein phosphatase 1, regulatory subunit 104; Unknown (protein for MGC:128845); XELAEV_18000877mg; XMCM7; xMCM7-A
• Gene Symbols: Mcm7
• Host Species: Mouse
• Immunogen: E.coli-derived human MCM7 recombinant protein (Position: D526-V719).
• Purification Method: Antigen affinity chromatography
• Quantity: 100 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 288532, 4176
• Target Species: Human, Rat
• Content And Storage: -20°C
• Product Type: Antibody
• Form: Lyophilized
• Isotype: IgG1