MilliporeSigma™ MCM9, Rabbit, Unlabeled, Polyclonal,

Rabbit Polyclonal Antibody

Supplier:  MilliporeSigma™ ABE2603

Catalog No. ABE2603

Description

DNA helicase MCM9 (EC 3.6.4.12; UniProt Q9NXL9; also known as hMCM9, Mini-chromosome maintenance deficient domain-containing protein 1) is encoded by the MCM9 (also known as C6orf61, MCMDC1, ODG4) gene (Gene ID 254394) in human. Mammalian minichromosome maintenance (MCM) protein homologues MCM8 and MCM9 play important roles in DNA replication elongation and prereplication complex (pre-RC) formation. MCM9 is found physically interacted with MCM8 and the interaction is essential for MCM9 cellular stability. MCM8-MCM9 complex is required for homologous recombination- (HR-) mediated repair of double-strand breaks (DSBs). In addition, the ATPase motifs of MCM8 and MCM9 (a.a. 523-530) are needed for MRN (MRE11-RAD50-NBS1) complex recruitment to DSBs sites. Cancer cells with MCM9 homozygous deletion display enhanced reduction in cell survival following exposure to interstrand-crosslinking (ICL) agents, such as cisplatin, due to impaired HR efficiency.

Specifications

• Antigen: MCM9
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: MCM9, C6orf61, MCMDC1, ODG4
• Host Species: Rabbit
• Purification Method: Unpurified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: This rabbit polyclonal antiserum detected DNA damage-induced MCM9 nuclear foci formation in U2OS and wild-type, but not Mcm9-null MEFs (Park, J., et al. (2013). Mol. Cell. Biol. 33(8):1632-1644). The C-terminal end epitope region is present in all four human MCM9 spliced isoforms and the first two mouse MCM9 isoforms reported by UniProt (Q9NXL9 and Q2KHI9).
• Content And Storage: Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

• Applications: Immunocytochemistry, Immunoprecipitation, Western Blot
• Concentration: Please refer to lot specific datasheet.
• Formulation: Rabbit polyclonal antibody serum with 0.05% Sodium Azide.
• Gene Accession No.: Q9NXL9
• Immunogen: Recombinant human MCM9 C-terminal fragment.
• Quantity: 100 μL
• Research Discipline: Epigenetics & Nuclear Function
• Gene ID (Entrez): NP_060166
• Target Species: Human
• Form: Serum

For Research Use Only. Not for use in diagnostic procedures.