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PeproTech MCP-1 Polyclonal Antibody, PeproTech®, Invitrogen™

Catalog No. 500P11350UG
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50 μg
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500P11350UG 50 μg
500P1131MG 1 mg
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Description

Rabbit Polyclonal Antibody

AA Sequence of recombinant protein: QPDAVNAPLT CCYSFTSKMI PMSRLESYKR ITSSRCPKEA VVFVTKLKRE VCADPKKEWV QTYIKNLDRN QMRSEPTTLF KTASALRSSA PLNVKLTRKS EANASTTFST TTSSTSVGVT SVTVN. Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Murine JE/MCP-1 (CCL2). Anti-Murine JE/MCP-1 (CCL2)-specific antibody was purified by affinity chromatography employing an immobilized Murine JE/MCP-1 (CCL2) matrix. Sandwich ELISA: To detect Murine JE/MCP-1 (CCL2) by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Murine JE/MCP-1 (CCL2) (500-P113Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Murine JE/MCP-1(CCL2). Western Blot: To detect Murine JE/MCP-1 (CCL2) by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Murine JE/MCP-1 (CCL2) is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

This gene is one of several cytokine genes clustered on the q-arm of chromosome 17. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The protein encoded by this gene is structurally related to the CXC subfamily of cytokines. Members of this subfamily are characterized by two cysteines separated by a single amino acid. This cytokine displays chemotactic activity for monocytes and basophils but not for neutrophils or eosinophils. It has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis and atherosclerosis. It binds to chemokine receptors CCR2 and CCR4.
TRUSTED_SUSTAINABILITY

Specifications

Antigen MCP-1
Applications ELISA, Western Blot
Classification Polyclonal
Concentration 0.1-1.0 mg/mL
Conjugate Unconjugated
Formulation PBS with no preservative
Gene Ccl2
Gene Accession No. P10148
Gene Alias Acidic seminal fluid protein; AI323594; C-C motif chemokine 2; C-C motif chemokine ligand 2; Ccl2; chemokine (C-C motif) ligand 2; GDCF-2; HC11; H-MCP-1; HSMCR30; immediate-early serum-responsive JE protein; immediate-early serum-responsive protein JE; Je; MCAF; Mcp1; MCP-1; MCP1A; MCP-1A; MGC9434; Monocyte chemoattractant protein 1; monocyte chemoattractant protein-1; monocyte chemotactic and activating factor; Monocyte chemotactic protein 1; monocyte chemotactic protein 1A; Monocyte secretory protein JE; monocyte-chemoattractant protein-1 precursor; platelet-derived growth factor-inducible protein JE; RP23-350G1.3; SCYA2; Sigje; small inducible cytokine A2; small inducible cytokine A2 (monocyte chemotactic protein 1, homologous to mouse Sig-je); small inducible cytokine subfamily A (Cys-Cys), member 2; small inducible gene JE; small-inducible cytokine A2; SMC-CF
Gene Symbols Ccl2
Host Species Rabbit
Immunogen E.coli-derived Recombinant Murine JE/MCP-1 (CCL2).
Purification Method Antigen affinity chromatography
Quantity 50 μg
Regulatory Status RUO
Primary or Secondary Primary
Gene ID (Entrez) 20296
Target Species Mouse
Content And Storage -20°C
Product Type Antibody
Form Lyophilized
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