MCP-4 Polyclonal Antibody, PeproTech®, Invitrogen™

Rabbit Polyclonal Antibody

Catalog Number	Mfr. No.	Quantity
500P0450UG	Invitrogen™ 500P0450UG	50 μg
500P04100UG	Invitrogen™ 500P04100UG	100 μg

Description

AA Sequence of recombinant protein: QPDALNVPST CCFTFSSKKI SLQRLKSYVI TTSRCPQKAV IFRTKLGKEI CADPKEKWVQ NYMKHLGRKA HTLKT. Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human MCP-4 (CCL13). Anti-Human MCP-4 (CCL13)-specific antibody was purified by affinity chromatography employing an immobilized Human MCP-4 matrix. Sandwich ELISA: To detect Human MCP-4 (CCL13) by sandwich ELISA (using 100 μL/well) a concentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human MCP-4 (CCL13) (500-P04BT) as a detection antibody, allows the detection of at least 2000- 4000 pg/mL of Recombinant Human MCP-4 (CCL13). Western Blot: To detect Human MCP-4 (CCL13) by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. When used in conjunction with compatible secondary reagents the detection limit for Recombinant Human MCP-4 (CCL13) is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Monocyte Chemotactic Protein 4 (MCP-4), also called CCL13, is induced by inflammatory proteins such as IL-1 and TNFalpha. MCP-4 is a ligand for three different G protein coupled receptors, CCR2, CCR3 and CCR5. MCP-4 activates signaling in monocytes, T lymphocytes, eosinophils and basophils and this signaling is associated with the allergic response.

Specifications

• Antigen: MCP-4
• Applications: ELISA, Western Blot
• Conjugate: Unconjugated
• Host Species: Rabbit
• Target Species: Human
• Gene Accession No.: Q99616
• Gene ID (Entrez): 6357
• Immunogen: E.coli-derived Recombinant Human MCP-4 (CCL13).
• Purification Method: Antigen affinity chromatography
• Gene: CCL13

• Concentration: 0.1-1.0 mg/mL
• Classification: Polyclonal
• Form: Lyophilized
• Regulatory Status: RUO
• Formulation: PBS with no preservative
• Gene Alias: C-C motif chemokine 13; C-C motif chemokine 13, long chain; C-C motif chemokine 13, medium chain; C-C motif chemokine 13, short chain; C-C motif chemokine ligand 13; CCL 13; CCL13; chemokine (C-C motif) ligand 13; CKb10; CK-beta-10; H-MCP-4; MCP4; MCP-4; Monocyte chemoattractant protein 4; monocyte chemotactic protein 4; NCC1; NCC-1; new CC chemokine 1; SCYA13; SCYL1; small inducible cytokine subfamily A (Cys-Cys), member 13; small-inducible cytokine A13
• Gene Symbols: CCL13
• Primary or Secondary: Primary
• Content And Storage: -20°C