Invitrogen™ MCU Monoclonal Antibody (CL3576)

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ MA524702

Catalog No. PIMA524702

Description

Immunogen sequence: TRQEYVYPEAR DRQYLLFFHK GAKKSRFDLE KYNQLKDAIA QAEMDLKRLR DPLQVHLPLR QIGE Binds to an epitope located within the peptide sequence RDRQYLLFFH as determined by overlapping synthetic peptides. Highest antigen sequence identity to the following orthologs - mouse 100%, rat 100%.

Mitochondrial inner membrane calcium uniporter that mediates calcium uptake into mitochondria. Constitutes the pore-forming and calcium-conducting subunit of the uniporter complex (uniplex). Activity is regulated by MICU1 and MICU2. At low Ca(2+) levels MCU activity is down-regulated by MICU1 and MICU2; at higher Ca(2+) levels MICU1 increases MCU activity. Mitochondrial calcium homeostasis plays key roles in cellular physiology and regulates cell bioenergetics, cytoplasmic calcium signals and activation of cell death pathways. Involved in buffering the amplitude of systolic calcium rises in cardiomyocytes. While dispensable for baseline homeostatic cardiac function, acts as a key regulator of short-term mitochondrial calcium loading underlying a 'fight-or-flight' response during acute stress: acts by mediating a rapid increase of mitochondrial calcium in pacemaker cells. participates in mitochondrial permeability transition during ischemia-reperfusion injury. Regulates glucose-dependent insulin secretion in pancreatic beta-cells by regulating mitochondrial calcium uptake. Mitochondrial calcium uptake in skeletal muscle cells is involved in muscle size in adults. Regulates synaptic vesicle endocytosis kinetics in central nerve terminal. Involved in antigen processing and presentation.

Specifications

• Antigen: MCU
• Classification: Monoclonal
• Concentration: 1.00 mg/mL
• Formulation: PBS with 40% glycerol and 0.02% sodium azide; pH 7.2
• Gene Accession No.: Q8NE86
• Gene Symbols: MCU
• Immunogen: Recombinant Human MCU.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG1

• Applications: Western Blot, Immunocytochemistry
• Clone: CL3576
• Conjugate: Unconjugated
• Gene: MCU
• Gene Alias: 2010012O16Rik; AV064928; C10orf42; Calcium uniporter protein, mitochondrial; Ccdc109a; coiled-coil domain containing 109A; coiled-coil domain-containing protein 109A; D130073L02Rik; Gm64; HsMCU; MCU; mitochondrial calcium uniporter
• Host Species: Mouse
• Purification Method: Protein A
• Regulatory Status: RUO
• Gene ID (Entrez): 90550
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid