Meis1/2, Rabbit anti-Mouse, Polyclonal, Millipore Sigma™

Rabbit Polyclonal Antibody

Catalog Number	Mfr. No.	Quantity
ABE2865100	MilliporeSigma ABE2865100UG	100 μg

Description

Anti-Meis1/2 Antibody, Cat. No. ABE2865, is a highly specific rabbit polyclonal antibody that targets Meis1/2 and has been tested in Chromatin Immunoprecipitation (ChIP), Immunofluorescence, and Western Blotting.

Homeobox protein Meis1 (UniProt: Q60954-1; also known as Myeloid ecotropic viral integration site 1) Homeobox protein Meis2 (UniProt: P97367-2; also known as Meis1-related protein 1) are encoded by the Meis1 and Meis2 (also known as Mrg1, Stra10) genes (Gene ID: 17268 and 17536) in murine species. Meis proteins act as transcription factors that are orthologous to the Drosophila homothorax (Hth) protein. They are expressed dynamically in several organs during embryogenesis and exert potent regulatory activity through their interaction with Hox proteins and other transcription factors. Meis protein contain a TALE (three-amino-acid loop extension) sub-class of the homeodomain that binds to DNA. They directly bind to Pbx proteins and the Meis/Pbx protein complex binds to DNA through respective Meis- and Pbx-consensus binding sites to regulate transcription. The Meis/Pbx complex plays important roles during development of multiple organs. In humans and mice, three homologues Meis1, Meis2 and Meis3 have been identified. Meis1 is expressed at high levels in the lung and at lower levels in the heart and brain. On the other hand, Meis 2 displays spatially restricted expression patterns in the developing nervous system, limbs, face, and in various viscera. In adult, it is mainly expressed in the brain and female genital tract, with a different distribution of the alternative splice forms in these organs. Pancreatic beta cells express both Meis1 and 2. Both Meis1 and 2 are expressed at high levels in all stages of embryonic development of mice (day 7 to 17). Meis3 is expressed in pancreatic islets and is reported to control the expression of expression of PDK1, which promotes survival of pancreatic beta-cells. Deficiency of Meis1 or Meis 2 can lead to embryonic lethality. Meis1-deficient mice die around midgestation due to a general failure of definitive hematopoiesis. (Ref.: Machon, O et al. (2015). BMC Dev. Biol. 515:40; Azcoitia, V et al., (2005). Dev. Biol. 280 (2); 307-320).

Specifications

• Antigen: Meis1/2
• Applications: ChIP Assay, Immunofluorescence, Western Blot
• Conjugate: Unconjugated
• Host Species: Rabbit
• Research Discipline: Epigenetics & Nuclear Function
• Formulation: Purified rabbit polyclonal antibody in PBS with 0.05% sodium azide and 50% glycerol.
• Gene ID (Entrez): NP_001180200;NP_001153039;NP_001129544
• Immunogen: KLH-conjugated linear peptide corresponding to 14 amino acids from the C-terminal region of murine Meis1a, Meis2a, and 13 amino acids from Meis2b isoforms.
• Purification Method: Protein A purified
• Test Specificity: This rabbit polyclonal antibody detects Meis1/2. It targets an epitope with in 14 amino acids from the C-terminal region.

• Dilution: Immunofluorescence Analysis: A representative lot detected Meis1/2 in Immunofluorescence applications (Mercader, N., et. al. (2005). Development. 132(18):4131-42; Marcos, S., et. al. (2015). Development. 142(17):3009-20).Chromatin Immunoprecipitation (ChIP) Analysis: A representative lot detected Meis1/2 in Chromatin Immunoprecipitation applications (Penkov, D., et. al. (2013). Cell Rep. 3(4):1321-33; Marcos, S., et. al. (2015). Development. 142(17):3009-20).Western Blotting Analysis: A representative lot detected Meis1/2 in Western Blotting applications (Mercader, N., et. al. (2005). Development. 132(18):4131-42).
• Classification: Polyclonal
• Form: Purified
• Regulatory Status: RUO
• Target Species: Mouse
• Gene Alias: Homeobox protein Meis1;Myeloid ecotropic viral integration site 1;Homeobox protein Meis2;Meis1-related protein 1
• Gene Symbols: Meis1;Meis2;Mrg1;Stra10
• Primary or Secondary: Primary
• Content And Storage: Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
• Molecular Weight of Antigen: ∽51 kDa observed; 43.00, 43.79, and 43.06 kDa for Meis1a, Meis2a, and Meis2b, respectively. Uncharacterized bands may be observed in some lysate(s).