Meis1, Rabbit anti-Human, Mouse, Polyclonal, Millipore Sigma™

Catalog No.	Quantity
ABE286425UG	25 μg

Catalog No. ABE286425UG Supplier MilliporeSigma Supplier No. ABE286425UG

Description

Rabbit Polyclonal Antibody

Anti-Meis1, Cat. No. ABE2864-25UG, is a highly specific rabbit polyclonal antibody, that targets Homeobox protein Meis1 and has been tested in Western Blotting, Chromatin Immunoprecipitation (ChIP) and Immunofluorescence.

Homeobox protein Meis1 (UniProt: Q60954-1 also known as Myeloid ecotropic viral integration site 1 (Meis1) and Meis1-related protein 1) is encoded by the Meis1 gene (Gene ID: 17268) Meis proteins act as transcription factors that are orthologous to the Drosophila homothorax (Hth) protein. Meis protein contain a TALE (three-amino-acid loop extension) sub-class of the homeodomain that binds to DNA. Meis protein directly bind to Pbx proteins and the Meis/Pbx protein complex binds to DNA through respective Meis- and Pbx-consensus binding sites to regulate transcription. The Meis/Pbx complex plays important roles during development of multiple organs. In humans and mice, three homologues Meis1, Meis2 and Meis3 have been identified. Meis1 is expressed at high levels in the lung and at lower levels in the heart and brain. On the other hand, Meis 2 displays spatially restricted expression patterns in the developing nervous system, limbs, face, and in various viscera. In adult, it is mainly expressed in the brain and female genital tract, with a different distribution of the alternative splice forms in these organs. Pancreatic beta cells express both Meis1 and 2. Both Meis1 and 2 are expressed at high levels in all stages of embryonic development of mice (day 7 to 17). Deficiency of Meis1 or Meis 2 can lead to embryonic lethality. (Ref.: Machon, O et al. (2015). BMC Dev. Biol. 515:40).

Specifications

• Antigen: Meis1
• Applications: ChIP Assay, Immunofluorescence, Western Blot
• Classification: Polyclonal
• Conjugate: Unconjugated
• Dilution: Western Blotting Analysis: 1μg/mL of this antibody detected Meis1 in 10μg of K562 cell lysate. Immunofluorescence Analysis: A representative lot detected Meis1 in adjacent stage (St) 41 sagittal hindbrain and eye sections (Mercader, N., et. al. (2005). Development. 132(18):4131-42). Western Blotting Analysis: A 1:800 dilution from a representative lot detected Meis1 in HEK293T cells transfectd with Meis1a (C ourtesy of Dr. Valeria Azcoitia and Dr. Miguel Torres ). Chromatin Immunoprecipitation (ChIP) Analysis: A representative lot detected Meis1 in Chromatin Immunoprecipitation applications (Penkov, D., et. al. (2013). Cell Rep. 3(4):1321-33). Western Blotting Analysis: A representative lot detected Meis1 in proximal, distal, and RA-treated distal blastemas (Mercader, N., et. al. (2005). Development. 132(18):4131-42).
• Formulation: Purified rabbit polyclonal antibody in PBS with 0.05% sodium azide and 50% glycerol.
• Gene Alias: Homebox protein Meis1
• Gene Symbols: Meis1
• Host Species: Rabbit
• Immunogen: KLH-conjugated linear peptide corresponding 14 amino acids from the C-terminal region of Meis1.
• Molecular Weight of Antigen: 52 kDa observed. Uncharacterized bands may be observed in some lysate(s).
• Purification Method: Protein A purified
• Quantity: 25 μg
• Regulatory Status: RUO
• Research Discipline: Epigenetics & Nuclear Function
• Primary or Secondary: Primary
• Test Specificity: This rabbit polyclonal antibody detects Meis1 in human and murine cells. It targets an epitope within the C-terminal region.
• Target Species: Human, Mouse
• Content And Storage: Stable for 1 year at -20°C from date of receipt.
• Form: Purified
• Isotype: IgG