MEK2 C-Term Monoclonal Antibody (12A6.G1.G11), Rockland™

Mouse Monoclonal Antibody

Supplier:  Rockland Immunochemicals 200301GQ2S

Catalog No. 50-199-0618

Description

Store vial at -20°C prior to opening. This product is stable at 4°C as an undiluted liquid. For extended storage, aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Dilute only prior to immediate use. Reacts specifically with human MEK2. Cross reactivity is expected to occur with human, mouse, and rat based on sequence identity of the peptide immunogen. This antibody does not react with the MEK1 isoform.

MEK1 (Mitogen activated protein kinase 1) catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. MEK1 activates ERK1 and ERK2 MAP kinases. Mitogen activated protein kinase 2 (MEK2 or MAPKK2) is a member of a family of tyrosine/threonine protein kinases that activate the ERK1 and 2 and MAPK enzymes by phosphorylating both residues within the threonine/glutamate/tyrosine (TEY) motif in the activation loop. MEK1 and 2 are also activated by dual phosphorylation, which occurs on serine 218 and 222, in the activation loop of the MEK. Threonine 292 of MEK1 is phosphorylated by ERK 2, which serves as a negative feedback loop by suppressing activation of MEK1.

Specifications

• Antigen: MEK2 C-Term
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: 0.02M potassium phosphate with 0.15M NaCl and 0.01% sodium azide; pH 7.2
• Gene Accession No.: P36506, P36507, Q63932
• Gene Symbols: MAP2K2
• Immunogen: Anti-MEK2 Monoclonal Antibody was produced in mice by repeated immunizations with synthetic peptide corresponding to amino acid residues near the C-terminus conjugated to KLH.
• Regulatory Status: RUO
• Gene ID (Entrez): 26396, 5605, 58960
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid

• Applications: ELISA, Western Blot
• Clone: 12A6.G1.G11
• Conjugate: Unconjugated
• Gene: MAP2K2
• Gene Alias: AA589381; CFC4; dual specificity mitogen activated protein kinase kinase 2; Dual specificity mitogen-activated protein kinase kinase 2; ERK activator kinase 2; ERK kinase 2; FLJ26075; MAP kinase kinase 2; MAP kinase/Erk kinase; MAP2K1; Map2k2; MAPK; MAPK/ERK kinase 1; MAPK/ERK kinase 2; MAPKK 1; MAPKK 2; MAPKK2; MEK 1; MEK 2; MEK-1; Mek2; MEK-2; mitogen activated protein kinase kinase 2; mitogen-activated protein kinase kinase 2; mitogen-activated protein kinase kinase 2, p45; MK2; MKK1; Mkk2; OTTHUMP00000165827; PRKMK1; Prkmk2; protein kinase, mitogen activated, kinase 2, p45
• Host Species: Mouse
• Quantity: 25 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG1 κ