Invitrogen™ MEKK3 Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA578058

Catalog No. PIPA578058

Description

Positive Control: 3xFlag-human MAP3K3-transfected 293T Predicted Reactivity: Rat (98%), Rhesus Monkey (100%) Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

MAP3K3 (MEKK3) directly regulates MAPK8 and extracellular signal-regulated protein kinase (ERK) pathways by phosphorylating multiple MAP2Ks. Northern blot analysis revealed a 4. 6-kb transcript that appears to be ubiquitously expressed. This protein directly regulates the stress-activated protein kinase (SAPK) and extracellular signal-regulated protein kinase (ERK) pathways by activating SEK and MEK1/2 respectively; it does not regulate the p38 pathway. In cotransfection assays, it enhanced transcription from a nuclear factor kappa-B (NFKB)-dependent reporter gene, consistent with a role in the SAPK pathway. Alternatively spliced transcript variants encoding distinct isoforms have been observed.

Specifications

• Antigen: MEKK3
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: Map3k3
• Gene Alias: AW548911; MAP/ERK kinase kinase 3; MAP3K3; MAPK/ERK kinase kinase 3; MAPKKK3; MEK kinase 3; MEKK 3; Mekk3; mitogen activated protein kinase kinase kinase 3; mitogen-activated protein kinase kinase kinase 3; mKIAA4031
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 26406, 4215
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Immunohistochemistry (Paraffin), Western Blot
• Concentration: 0.71 mg/mL
• Formulation: 0.1M tris glycine with 10% glycerol and 0.01% thimerosal; pH 7
• Gene Accession No.: Q61084, Q99759
• Gene Symbols: Map3k3
• Immunogen: Recombinant protein encompassing a sequence within the center region of human MEKK3. The exact sequence is proprietary.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse
• Product Type: Antibody
• Isotype: IgG

Safety and Handling

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