Invitrogen™ MELK Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA5110824

Catalog No. PIPA5110824

Description

Immunogen sequence: DIKSNNWSLE DVTASDKNYV AGLIDYDWCE DDLSTGAATP RTSQFTKYWT ESNGVESKSL TPALCRTPAN KLKNKENVYT PKSAVKNEEY FMFPEPKTPV NKNQHKREIL TTPNRYTTPS KARNQCLKET PIKIPVNSTG TD.

MELK is a serine/threonione kinase which is related to the AMPK family that has been linked to the cell cycle. MELK has also been shown to be overexpressed in cancer and has been identified has a potential target for cancer therapeutics. MELK has a broad substrate specificity; phosphorylates BCL2L14, CDC25B, MAP3K5/ASK1 and ZNF622. Acts as an activator of apoptosis by phosphorylating and activating MAP3K5/ASK1. Acts as a regulator of cell cycle, notably by mediating phosphorylation of CDC25B, promoting localization of CDC25B to the centrosome and the spindle poles during mitosis. Plays a key role in cell proliferation and carcinogenesis. Required for proliferation of embryonic and postnatal multipotent neural progenitors. Phosphorylates and inhibits BCL2L14, possibly leading to affect mammary carcinogenesis by mediating inhibition of the pro-apoptotic function of BCL2L14. Also involved in the inhibition of spliceosome assembly during mitosis by phosphorylating ZNF622, thereby contributing to its redirection to the nucleus. May also play a role in primitive hematopoiesis.

Specifications

• Antigen: MELK
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: MELK
• Gene Alias: AI327312; hMELK; HPK38; Kiaa0175; Maternal embryonic leucine zipper kinase; Melk; mKIAA0175; MPK38; OTTHUMP00000046113; pEg3 kinase; Pk38; Protein kinase Eg3; protein kinase PK38; RP11-8N6.1; Tyrosine-protein kinase MELK
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 9833
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Immunohistochemistry (Paraffin), Western Blot
• Concentration: 0.1 mg/mL
• Formulation: PBS with 40% glycerol and 0.02% sodium azide; pH 7.2
• Gene Accession No.: Q14680
• Gene Symbols: MELK
• Immunogen: Recombinant Protein corresonding to Human MELK. Recombinant protein control fragment (Product #RP-89807).
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov