Gibco™ MEM, no glutamine

Minimum Essential Medium (MEM) is one of the most commonly used of all cell culture media. MEM can be used with a variety of suspension and adherent mammalian cells.

Catalog Number	Mfr. No.	Quantity
11-090-081	Gibco™ 11090081	500 mL
11-090-099	Gibco™ 11090099	10 x 500 mL
11-090-073	Gibco™ 11090073	1000 mL
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Description

Minimum Essential Medium (MEM) is one of the most commonly used of all cell culture media. MEM can be used with a variety of suspension and adherent mammalian cells, including HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, fibroblasts, and primary rat astrocytes. We offer a variety of Gibco™ MEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This MEM is modified as follows:
With: Phenol Red
Without: L-glutamine, HEPES

The complete formulation is available.

Gibco™ MEM, developed by Harry Eagle, was based on his earlier formulation of Basal Medium Eagle (BME). Many other modifications of MEM followed, including Glasgow’s MEM, MEM α, DMEM, and Temin’s Modification. MEM is available with Earle’s salts for use in a CO₂ incubator, or with Hanks' salts for use without CO₂. This product is made with Earle’s salts.

MEM contains no proteins, lipids, or growth factors. Therefore, MEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). MEM uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5–10% CO₂ environment to maintain physiological pH.

Specifications

• Cell Line: HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, and fibroblasts
• Classification: Animal Origin-free
• Form: Liquid
• Serum Level: Standard Serum Supplementation
• With Additives: Low Glucose, Phenol Red
• Green Features: Sustainable packaging
• Product Line: Gibco™

• Cell Type: Primary Rat Astrocytes
• Concentration: 1 X
• Product Type: MEM (Minimum Essential Medium)
• Sterility: Sterile-filtered
• Without Additives: No Glutamine, No HEPES, No Sodium Pyruvate
• Manufacturing Quality: cGMP-compliant under the ISO 13485 standard

Safety and Handling

ShelfLife : 24 Months From Date of Manufacture

For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.