Invitrogen™ Human Mer (MERTK) ELISA Kit

Description

ELISA

The Human Mer ELISA Kit is an enzyme-linked immunosorbent assay for the quantitative detection of human Mer.

MERTK (cMER) is a tyrosine kinase proto-oncogene and is involved in the retinal pigment epithelium (RPE) phagocytosis pathway, which is implicated in human retinal disease. Regulates many physiological processes including cell survival, migration, differentiation, and phagocytosis of apoptotic cells (efferocytosis). Ligand binding at the cell surface induces autophosphorylation of MERTK on its intracellular domain that provides docking sites for downstream signaling molecules. Following activation by ligand, interacts with GRB2 or PLCG2 and induces phosphorylation of MAPK1, MAPK2, FAK/PTK2 or RAC1. MERTK signaling plays a role in various processes such as macrophage clearance of apoptotic cells, platelet aggregation, cytoskeleton reorganization and engulfment. Functions in the retinal pigment epithelium (RPE) as a regulator of rod outer segments fragments phagocytosis. Plays also an important role in inhibition of Toll-like receptors (TLRs)-mediated innate immune response by activating STAT1, which selectively induces production of suppressors of cytokine signaling SOCS1 and SOCS3.

Specifications

• Accession Number: c-Eyk; c-mer; c-mer proto-oncogene tyrosine kinase; Eyk; Mer; MER proto-oncogene, tyrosine kinase; MER receptor tyrosine kinase; MERTK; MGC133349; nmf12; Nyk; Proto-oncogene c-Mer; proto-oncogene tyrosine-protein kinase MER; rdy; Receptor tyrosine kinase MerTK; Receptor tyrosine tinase gene probably the gene for Rdy; retinal dystrophy; RP38; sMER; sMERTK; soluble MER; soluble MERTK; STK kinase; Tyro 12; Tyro12; Tyrosine-protein kinase Mer
• Assay Range: 11.72 to 750 pg/mL
• Assay Sensitivity: 6.96 pg/mL
• Conjugate: HRP
• Product Type: ELISA
• Sample Type: Cell Lysates, Plasma, Serum
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene ID (Entrez): 10461
• Gene Symbol: Mertk
• Interassay CV: 7.4%
• Intraassay CV: 3.9
• Kit Contents: Pre-coated 96 well plate, Standard (2 vials), Assay Buffer concentrate, Biotinylated Detection Antibody, Streptavidin-HRP, Wash Buffer, Substrate Solution, Stop Solution, Adhesive Plate Covers
• Label or Dye: HRP
• Quantity: 10 x 96 Tests
• Regulatory Status: RUO
• Sample Volume: Cell Lysate 5 μL, Plasma 5 μL, Serum 5 μL
• Storage Requirements: 2°C to 8°C
• Target: MERTK
• Test Time: 1 hrs 20 mins
• Total Assay Time: 3 hrs 30 mins