Thermo Scientific Chemicals MES, 0.5M buffer soln., pH 5.0

Description

CAS: 145224-94-8 | C6H15NO5S | 213.248 g/mol

MES, is often used as a running buffer for resolving very small proteins on bis-tris gels, also used as a biological buffer in plant cell cultures. It is also been used in various protein characterization assays. This sterile solution suitable for cell culture and molecular biology applications. It is also used as a buffering agent in biology and biochemistry.

This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.

Applications
MES, is often used as a running buffer for resolving very small proteins on bis-tris gels, also used as a biological buffer in plant cell cultures. It is also been used in various protein characterization assays. This sterile solution suitable for cell culture and molecular biology applications. It is also used as a buffering agent in biology and biochemistry.

Solubility
Soluble in water (0.5 M at 20°C).

Notes
Solutions are stable at 2-8°C for months. Sterilize by filtration through 0.2 mm filters. Autoclaving is not recommended for any sulfonic acid buffer. If buffers must be nuclease-free, treat the water first, then add the buffer after autoclaving. When MES solutions are autoclaved, they turn yellow (although pH does not change measurably). The identity of the yellow breakdown product is unknown.

Specifications

Chemical Identifiers

• CAS: 145224-94-8
• Molecular Formula: C6H15NO5S
• Molecular Weight (g/mol): 213.248
• MDL Number: MFCD00283848
• InChI Key: MIIIXQJBDGSIKL-UHFFFAOYSA-N
• PubChem CID: 16218417
• IUPAC Name: 2-morpholin-4-ylethanesulfonic acid;hydrate
• SMILES: C1COCCN1CCS(=O)(=O)O.O

• Color: Colorless
• Concentration: 0.5 M
• pH: 5.0
• Physical Form: Liquid
• Quantity: 100 mL
• Solubility Information: Soluble in water (0.5 M at 20°C).
• Chemical Name or Material: MES buffer soln.

Safety and Handling

Hazard Category	Warning
Hazard Statement	GHS H Statement H315-H319 Causes skin irritation. Causes serious eye irritation.
Precautionary Statement	GHS P Statement P280-P305+P351+P338-P362-P321-P332+P313-P337+P313 Wear protective gloves/protective clothing/eye protection/face protection. IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. Take off contaminated clothing and wash before reuse. Specific treatment (see label). If skin irritation occurs: Get medical advice/attention. If eye irritation persists: Get medical advice/attention.
TSCA	Yes
Recommended Storage	Ambient temperatures

SDS

RUO – Research Use Only