Invitrogen™ Mesothelin Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA5144122

Catalog No. FEPA5144122

Description

Mesothelin Polyclonal Antibody for ICC/IF, IHC (P), IP, ELISA

Originally identified as a differentiation antigen of mesotheliomas, ovarian cystadenocarcinomas, and pancreatic adenocarcinomas, Mesothelin is a glycosylphosphatidylinositol (GPI)-anchored, cell-surface glycoprotein predominantly secreted by cells of the mesothelium. Although Mesothelin is expressed at restricted levels by normal mesothelial cells of the pleural,pericardial, and peritoneal membranes, aberrant expression has been documented in the aforementioned cancers, as well as in endometriod uterine adenocarcinomas and squamous cell carcinomas of the esophagus, stomach, lung, and cervix. Proteolytic cleavage of Mesothelin yields a soluble, polypeptide fragment designated megakaryocyte potentiating factor (MPF) based on its ability to stimulate megakaryocyte colony-forming activity of murine interleukin-3 in murine bone marrow cell cultures. Originally isolated from the HPC-Y5 pancreatic cell line, MPF has been suggested to play a role in the proliferation and differentiation of megakaryoctyes, and the regulation of resultant platelet production. While the biological functions of both Mesothelin and MPF remain speculative, high-levels of expression in cancerous tissues compared to limited distribution in normal tissues strongly suggests their involvement in tumorigenesis. Both have been demonstrated to promote tumor cell proliferation, migration,anchorage-independent growth, and tumor progression; demonstrating their involvement in heterotypic cell adhesion and the metastatic spread of cancer.

Specifications

• Antigen: Mesothelin
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: MSLN
• Gene Alias: CAK1; CAK1 antigen; megakaryocyte potentiating factor; Megakaryocyte-potentiating factor; Mes; Mesothelin; Mesothelin, cleaved form; MPF; MSLN; Pre-pro-megakaryocyte-potentiating factor; SMR; SMRP; soluble MPF mesothelin related protein
• Host Species: Rabbit
• Purification Method: Protein G
• Regulatory Status: RUO
• Gene ID (Entrez): 10232
• Content And Storage: -20°C or -80°C if preferred
• Form: Liquid

• Applications: ELISA, Immunohistochemistry (Paraffin), Immunoprecipitation, Immunocytochemistry
• Concentration: 3.5 mg/mL
• Formulation: PBS with 50% glycerol and 0.03% Proclin 300; pH 7.4
• Gene Accession No.: Q13421
• Gene Symbols: MSLN
• Immunogen: Recombinant Human Mesothelin protein (37-598AA).
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG