Invitrogen™ Mesothelin Recombinant Rabbit Monoclonal Antibody (1I1Y7)

Rabbit Recombinant Monoclonal Antibody

Supplier:  Invitrogen™ MA551345

Catalog No. FEMA551345

Description

Immunogen sequence: EVEKTACPSG KKAREIDESL IFYKKWELEA CVDAALLATQ MDRVNAIPFT YEQLDVLKHK LDELYPQGYP ESVIQHLGYL FLKMSPEDIR KWNVTSLETL KALLEVNKGH EMSPQAPRRP LPQVATLIDR FVKGRGQLDK DTLDTLTAFY PGYLCSLSPE ELSSVPPSSI WAVRPQDLDT CDPRQLDVLY PKARLAFQNM NGSEYFVKIQ SFLGGAPTED LKALSQQNVS MDLATFMKLR TDAVLPLTVA EVQKLLGPHV EGLKAEERHR PVRDWILRQR QDDLDTLGLG LQGGIPNGYL VLDLSMQEAL S.

Originally identified as a differentiation antigen of mesotheliomas, ovarian cystadenocarcinomas, and pancreatic adenocarcinomas, Mesothelin is a glycosylphosphatidylinositol (GPI)-anchored, cell-surface glycoprotein predominantly secreted by cells of the mesothelium. Although Mesothelin is expressed at restricted levels by normal mesothelial cells of the pleural,pericardial, and peritoneal membranes, aberrant expression has been documented in the aforementioned cancers, as well as in endometriod uterine adenocarcinomas and squamous cell carcinomas of the esophagus, stomach, lung, and cervix. Proteolytic cleavage of Mesothelin yields a soluble, polypeptide fragment designated megakaryocyte potentiating factor (MPF) based on its ability to stimulate megakaryocyte colony-forming activity of murine interleukin-3 in murine bone marrow cell cultures. Originally isolated from the HPC-Y5 pancreatic cell line, MPF has been suggested to play a role in the proliferation and differentiation of megakaryoctyes, and the regulation of resultant platelet production. While the biological functions of both Mesothelin and MPF remain speculative, high-levels of expression in cancerous tissues compared to limited distribution in normal tissues strongly suggests their involvement in tumorigenesis. Both have been demonstrated to promote tumor cell proliferation, migration,anchorage-independent growth, and tumor progression; demonstrating their involvement in heterotypic cell adhesion and the metastatic spread of cancer.

Specifications

• Antigen: Mesothelin
• Classification: Recombinant Monoclonal
• Concentration: 0.58 mg/mL
• Formulation: PBS with 0.05% BSA, 50% glycerol and 0.05% ProClin 300; pH 7.3
• Gene Accession No.: Q13421
• Gene Symbols: MSLN
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 296-606 of human Mesothelin (NP_037536.2).
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Format: Liquid

• Applications: ELISA, Immunohistochemistry (Paraffin), Western Blot
• Clone: 1I1Y7
• Conjugate: Unconjugated
• Gene: MSLN
• Gene Alias: CAK1; CAK1 antigen; megakaryocyte potentiating factor; Megakaryocyte-potentiating factor; Mes; Mesothelin; Mesothelin, cleaved form; MPF; MSLN; Pre-pro-megakaryocyte-potentiating factor; SMR; SMRP; soluble MPF mesothelin related protein
• Host Species: Rabbit
• Purification Method: Affinity Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 10232
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid
• Isotype: IgG