Invitrogen™ Metadherin Recombinant Rabbit Monoclonal Antibody (21H9L18)

Catalog No.	Quantity
PI701171	100 μg

Catalog No. PI701171 Supplier Invitrogen™ Supplier No. 701171

Description

Rabbit Recombinant Monoclonal Antibody

This antibody is predicted to react with non-human primate, mouse and rat based on sequence homology. Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain. Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

Metadherin (Metastasis adhesion protein), also known as MTDH, LYsine-RIch CEACAM1 co-isolated (LYRIC), is a novel protein that localizes with the tight junction proteins ZO-1 and occludin in polarized epithelial cells. At the tight junction, it acts not as a structural component, but is rather recruited during the maturation of the tight junction complex. Metadherin is overexpressed in breast cancer tissue and breast tumor xenografts, while much lower levels are expressed in normal breast tissue. Metadherin binds to lung vasculature, one of the four common sites of breast cancer metastasis, through a C-terminal segment in the extracellular domain; blocking this lung-homing domain with antibodies or inhibiting metadherin with siRNA has been reported to inhibit breast cancer metastasis.

Specifications

• Antigen: Metadherin
• Applications: Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry
• Classification: Recombinant Monoclonal
• Clone: 21H9L18
• Concentration: 0.5 mg/mL
• Conjugate: Unconjugated
• Formulation: PBS with 0.09% sodium azide; pH 7.2-7.4
• Gene: MTDH
• Gene Accession No.: Q80WJ7, Q86UE4
• Gene Alias: 2610103J23Rik; 3D3; 3D3/Lyric; AEG1; AEG-1; astrocyte elevated gene 1; astrocyte elevated gene-1 protein; AV353288; D8Bwg1112e; LYRIC; LYRIC/ MTDH; LYRIC/3D3; lysine-rich CEACAM1 co-isolated protein; Metadherin; metastasis adhesion protein; MTDH; Protein LYRIC
• Gene Symbols: MTDH
• Host Species: Rabbit
• Immunogen: Peptide corresponding to amino acids 561-μ0 of human Metadherin (C-term).
• Purification Method: Protein A
• Quantity: 100 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 67154, 92140
• Target Species: Human, Mouse
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov