Methyl-Glutamine, Rabbit anti-All, Polyclonal, Millipore Sigma™

Rabbit Polyclonal Antibody

Supplier:  MilliporeSigma ABS218525UL

Catalog No. ABS218525UL

Description

Anti-Methyl-Glutamine, Cat. No. ABS2185, is a rabbit polyclonal antibody that detects glutamine methylated proteins. It has been tested for use in Western Blotting.

Glutamine methylation occurs on translation termination factors and ribosomal proteins. Protein methylation has been reported to affect various cellular processes including protein stability, protein-protein interaction, and protein localization. Methylation of histone is suggested as a platform to recruit and regulate other chromatin-related factors. Glutamine methylation is brought about by the action of protein glutamine methyltransferase HEMK2 that specifically requires a GQX3R motif for methylation activity. This antibody was tested for glutamine methylation in chromodomain helicase DNA-binding protein 5 (CHD5) that contains multiple PHD and chromo domains and is reported to function as a tumor suppressor.

Specifications

• Antigen: Methyl-Glutamine
• Classification: Polyclonal
• Dilution: Western Blotting Analysis: A 1:3,000 dilution from a representative lot detected Methyl-Glutamine in CHD5 unmethylated & CHD5 methylated recombinant lysate (Coutesy of, Dr. Kusevic, Dr. Kuditipudi and Dr. Jeltsch at University Stuttgart, Germany ). Western Blotting Analysis: A 1:10,000 dilution from a representative lot detected Methyl-Glutamine in in-vitro methylated CHD5, NUT, and eRF1 proteins (Kusevic, D., et. al. (2016). J Biol Chem. 291(12):6124-33).
• Host Species: Rabbit
• Molecular Weight of Antigen: ∽51 kDa observed (for methylated CHD5). Uncharacterized bands may be observed in some lysate(s).
• Quantity: 25 μL
• Research Discipline: Signaling
• Test Specificity: This rabbit polyclonal antibody detects methylglutamine in proteins.
• Content And Storage: Stable for 1 year at -20°C from date of receipt.

• Applications: Western Blot
• Conjugate: Unconjugated
• Formulation: Rabbit polyclonal antiserum with 0.05% sodium azide.
• Immunogen: KLH-conjugated Gly-Gln (Me)-Gly tripeptide.
• Purification Method: Unpurified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Target Species: All species
• Isotype: IgG