Methyl-Histone H3 (Arg2, Lys4)Rabbit anti-C. elegans, Chicken, Drosophila, Human, Mouse, Plant, Rat, Xenopus laevis, Polyclonal, Rockland™

Rabbit Polyclonal Antibody

Supplier:  Rockland Immunochemicals 600401I85

Catalog No. 50-199-2186

Description

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Anti-Histone H3 [Sym-dimethyl Arg2, Dimethyl Lys4] reacts with human Histone H3.2. A BLAST analysis was used to suggest cross-reactivity with Human, mouse, and C. elegans. Predicted to react with many species including rat, chicken, Xenopus, Drosophila, and plant based on 100% sequence homology. Cross-reactivity with Histone H3 from other sources has not been determined.

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in a histone cluster on chromosome 1. This gene is one of four histone genes in the cluster that are duplicated; this record represents the telomeric copy.

Specifications

• Antigen: Methyl-Histone H3 (Arg2, Lys4)
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: H3C15
• Gene Symbols: H3C15, H3C2
• Quantity: 50 μg
• Primary or Secondary: Primary
• Target Species: C. elegans, Chicken, Drosophila, Human, Mouse, Plant, Rat, X. laevis
• Product Type: Antibody

• Applications: ChIP Assay, Dot Blot, Immunofluorescence, Immunohistochemistry, Western Blot
• Concentration: 0.91 mg/mL
• Formulation: 0.02M potassium phosphate with 0.15M NaCl, 30% glycerol and 0.01% sodium azide; pH 7.2
• Gene Accession No.: P84228, Q71DI3
• Host Species: Rabbit
• Regulatory Status: RUO
• Gene ID (Entrez): 126961, 319150, 333932, 8358, 97114
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid