Mff, Rabbit anti-Human, Polyclonal, Millipore Sigma™

Rabbit Polyclonal Antibody

Supplier:  MilliporeSigma ABS208725UL

Catalog No. ABS208725UL

Description

Anti-Mff, Cat. No. ABS2087, is a rabbit polyclonal antibody that detects Mitochondrial fission factor (Mff) and has been tested for use in Immunocytochemistry, Immunofluorescence, and Western Blotting.

Mitochondrial fission factor (UniProt: Q9GZY8; also known as Mff) is encoded by the MFF (also known as C2orf33, AD030, AD033, GL004) gene (Gene ID: 56947) in human. Mff is a single-pass type IV membrane protein that is highly expressed in heart, kidney, liver, brain, muscle, and stomach. Mff is a homodimeric protein, but may also interact with other proteins. It plays a role in mitochondrial and peroxisomal fission and promotes the recruitment and association of the fission mediator dynamin-related protein 1 (DNM1L) to the mitochondrial surface. It is also suggested to be involved in regulation of synaptic vesicle membrane dynamics by recruitment of DNM1L to clathrin-containing vesicles. Mff has a cytoplasmic domain (aa 1-322), a helical domain (aa 323-340), and a mitochondrial intermembrane domain (aa 341-342). Mff is shown to be an essential factor for mitochondrial recruitment of Drp1 during mitochondrial fission in mammalian cells and its down-regulation inhibits mitochondrial recruitment of Drp1 and induces mitochondria elongation. Also, exogenous expression of Mff is shown to induce extensive mitochondrial fission. Mutations in MFF gene can cause a form of encephalopathy that is characterized by delayed psychomotor development, severe hypotonia with inability to walk, and microcephaly. (Ref.: Otera, H., et al. (2010). J. Cell Biol. 191 (6); 1141 1158; Gandre-Babbe, S., and van der Bliek, AM. (2008). Mol. Biol. Cell. 19(6): 2402-2412).

Specifications

• Antigen: Mff
• Classification: Polyclonal
• Dilution: Western Blotting Analysis: A representative lot detected Mff in Western Blotting applications (Mishra, P., et. al. (2015). Cell Metab. 22(6):1033-44).Immunofluorescence Analysis: A representative lot detected Mff in Immunofluorescence applications (Mishra, P., et. al. (2015). Cell Metab. 22(6):1033-44).
• Gene Alias: Mitochondrial fission factor
• Host Species: Rabbit
• Molecular Weight of Antigen: 38.47 kDa calculated.
• Quantity: 25 μL
• Research Discipline: Signaling
• Test Specificity: This rabbit polyclonal antibody detects mitochondrial fission factor (Mff) in human cells.
• Content And Storage: Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

• Applications: Immunocytochemistry, Immunofluorescence, Western Blot
• Conjugate: Unconjugated
• Formulation: Rabbit polyclonal antiserum with 0.05% sodium azide.
• Gene Symbols: MFF;C2orf33;AD030;AD033;GL004
• Immunogen: His-taged full length human recombinant mitochondrial fission factor (Mff).
• Purification Method: Unpurified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Target Species: Human
• Isotype: IgG