Invitrogen™ mGluR5 Monoclonal Antibody (N75/33)

Catalog No.	Quantity
PIMA527690	100 μg

Catalog No. PIMA527690 Supplier Invitrogen™ Supplier No. MA527690

Description

Mouse Monoclonal Antibody

1 μg/mL of MA5-27690 was sufficient for detection of mGluR1/5 glutamate receptor in 20 μg of rat brain membrane lysate and assayed by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody. Detects approximately 130kDa (both mGluR1 and mGluR5). This antibody was formerly sold as clone S75-33.

GRK1 is a member of the guanine nucleotide protein-coupled (G protein) receptor kinase subfamily of Serine/Threonine kinase family. GRK1 phosphorylates rhodopsin and initiates its deactivation. G protein mediated phosphorylation promotes the binding of arrestin, which removes the receptor from the G-protein and terminates the receptor signal chain. GRK1 is farnesylated to promote membrane association. Studies have shown that GRK1 has been shown to be inhibited by recoverin, a Calcium binding protein. Diseases associated with GRK1 protein dysfunction include Oguchi Disease-2 and Hereditary Night Blindness.

Specifications

• Antigen: mGluR5
• Applications: Immunohistochemistry (PFA fixed), Immunoprecipitation, Western Blot, Immunocytochemistry
• Classification: Monoclonal
• Clone: N75/33
• Concentration: 1 mg/mL
• Conjugate: Unconjugated
• Formulation: PBS with 50% glycerol and 0.09% sodium azide; pH 7.4
• Gene: Grm5
• Gene Accession No.: P31424, P41594, Q3UVX5
• Gene Alias: 6430542K11Rik; A830007B11Rik; AI850523; D16Ium24; D16Ium24e; G protein coupled receptor, family C, group 1, member E; G protein-coupled receptor GRM5; G protein-coupled receptor, family C, group 1, member E; Glu5R; Glur5; Glur-5; Glurbeta1; glutamate metabotropic receptor 5; glutamate receptor; glutamate receptor, metabotropic 5; GPRC1A; GPRC1E; GRM 1; GRM 5; GRM1; GRM1 alpha; GRM1A; Grm5; metabotropic 1; metabotropic glutamate receptor (mGluR5); metabotropic glutamate receptor 5; metabotropic glutamate receptor 5b; metabotropic glutamate receptor subtype 5b; metabotropic glutamate receptor type 5; MGC25101; mGlu1; mGlu5; mGluR; mGluR1; mGluR1 alpha; mGluR1A; MGLUR5; mGluR5a; mGluR5b; PPP1R86; protein phosphatase 1, regulatory subunit 86
• Gene Symbols: Grm5
• Host Species: Mouse
• Immunogen: Fusion protein amino acids 824-1203 (cytoplasmic C-terminus) of rat mGluR5b.
• Purification Method: Protein G
• Quantity: 100 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 108071, 24418, 2915
• Target Species: Human, Mouse, Rat
• Content And Storage: -20°C
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG1