Invitrogen™ MHC Class I Chimeric Recombinant Rabbit Monoclonal Antibody (R1-21.2)

Rabbit Recombinant Monoclonal Antibody

Supplier:  Invitrogen™ MA548095

Catalog No. PIMA548095

Description

Specificity: This antibody is specific for murine MHC class I, and binds to polymorphic H-2 determinants expressed on most haplotypes. This antibody is, therefore, able to bind all H-2 class I molecules, independent of the allele. This antibody inhibits the binding of most mouse anti-H-2 antibodies to H-2k cells.

Like their mammalian counterparts, avian MHC Class I molecules, also known as B-F antigens, consist of a highly polymorphic alpha-chain noncovalently bound to the invariant beta2-microglobulin subunit. MHC Class I molecules are expressed on most nucleated cells where they present endogenously synthesized antigenic peptides to CD8+ T lymphocytes, which are usually cytotoxic T cells. The monoclonal antibody F21-2 also reacts with turkey MHC Class I. HLA and MHC antibodies play a significant role in Immunopeptidomics, facilitating the identification and characterization of neoantigens through high-performance liquid chromatography coupled to tandem Mass Spectrometry.

Specifications

• Antigen: MHC Class I Chimeric
• Classification: Recombinant Monoclonal
• Concentration: 1 mg/mL
• Formulation: PBS with 0.02% ProClin 300
• Host Species: Rabbit
• Purification Method: Protein A
• Regulatory Status: RUO
• Target Species: Mouse
• Product Type: Antibody
• Isotype: IgG κ

• Applications: Immunohistochemistry, Immunoprecipitation, Radioimmune Assays (RIA), Western Blot, Immunocytochemistry
• Clone: R1-21.2
• Conjugate: Unconjugated
• Gene Alias: A29 protein; alpha chain 2; antigen presentation; antigen presentation; BFIV major; BF12 major; antigen presenting molecule; Aw-33; Aw-74; B12; BF; B-F; B-F antigen; G-B1; B-F histocompatibility F10 antigen; B-F major; B-F minor; BF1; B-F1; BF12; BF2; BFa1; BFa2; B-F-beta-IV; B-FI; BFIV; B-FIV; BFIV21; B-F-S01; B-F-S02; B-F-S03; B-F-S04; B-F-S05; B-F-S06; B-F-S07; B-F-S09; B-F-S11; B-F-S12; B-F-S13; B-F-S14; BFw-03; BFw-05; BFz-01; B-LB2; BOLA class I histocompatibility antigen, alpha chain BL3-7-like; chicken MHC class I antigen; class I histocompatibility antigen, F10 alpha chain; EA1.2; EA2.1; F10 alpha chain; HFE; HLA A; HLA class I antigen; HLA class I histocompatibility antigen, A alpha chain; HLA class I histocompatibility antigen, A-1 alpha chain; HLA class I histocompatibility antigen, A-30 alpha chain; HLA class I histocompatibility antigen, A-33 alpha chain; HLA class I histocompatibility antigen, A-74 alpha chain; HLA class I histocompatibility antigen, alpha chain E; HLA class I histocompatibility antigen, E alpha chain; HLA locus; HLA-6.2; HLAA; HLA-A; HLA-A locus alpha 2 domain; HLA-A null; HLA-A11; HLA-A33; HLA-Aw33.1; HLA-DQB1; HLA-DRB1; HLAE; HLA-E; Human leukocyte antigen A; leukocyte antigen class I-A; lymphocyte antigen; major class I glycoprotein; Major histocompatibility complex class I antigen BF2; major histocompatibility complex class I glycoprotein; major histocompatibility complex class I glycoprotein haplotype B21; major histocompatibility complex, class I, A; major histocompatibility complex, class I, E; major histocompatibility complex, class I, G; MCH class I antigen; MHC; MHC BF1 class I; MHC BF2 class I; MHC class 1 antigen; MHC class I; MHC class I alpha chain 1; MHC class I alpha chain 2; MHC class I anti; MHC Class I antigen; MHC class I antigen A*30; MHC class I antigen A*33; MHC class I antigen A*74; MHC class I antigen alpha chain; MHC class I antigen B-F heavy chain; MHC class I antigen B-F major heavy chain; MHC class I antigen B-F minor heavy chain; MHC class I antigen E; MHC class I antigen heavy chain; MHC class I antigen HLA-A heavy chain; MHC class I antigen HLA-A33; MHC class I antigen null protein; MHC class I antigene; MHC class I B-F heavy chain; MHC class I glycoprotein; MHC class I heavy chain B-F; MHC class I molecule; MHC class I-alpha; MHC class II antigen; MHC class1 antigen; MHC classI antigen; MHC HLA-E alpha-1; MHC HLA-E alpha-2.1; minor antigen; minor class I glycoprotein; OLA-I; OVAR; Ovar-I; ovar-MHCI-B564; ovar-MHCI-D5; ovar-MHCI-F12; put. F10 preprotein (AA -22 to 323); QA1; sHLA-E; Soluble HLA class I histocompatibility antigen, alpha chain E; truncated MHC class I antigen
• Immunogen: This antibody was raised by immunising Lewis 1F rats with mouse CH-1 lymphoma cells. Spleen cells from immunised rats were fused with X-63Ag8-653 myeloma cells to generate hybridomas.
• Quantity: 200 μg
• Primary or Secondary: Primary
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid