MHC Class I (H-2Db) Monoclonal Antibody (28-14-8), Functional Grade, eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

Supplier:  Invitrogen 16599982

Catalog No. 50-146-83

Description

Description: The 28-14-8 monoclonal antibody reacts with the mouse MHC class I, H-2D^b, and cross-reacts with H-2L^d, H-2D^q and/or H-2L^q. Binding of 28-14-8 to the alpha 3 domain of H-2L^d is not dependent on beta2-microglobulin. Applications Reported: This 28-14-8 antibody has been reported for use in flow cytometric analysis. Applications Tested: This 28-14-8 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Storage and handling: Use in a sterile environment. Filtration: 0.2 μm post-manufacturing filtered. Purity: Greater than 90%, as determined by SDS-PAGE. Endotoxin Level: Less than 0.001 ng/μg antibody, as determined by LAL assay. Aggregation: Less than 10%, as determined by HPLC.

This protein is an MHC class I alloantigen, which is involved in antigen presentation to T cells expressing CD3/TCR and CD8. HLA and MHC antibodies play a significant role in Immunopeptidomics, facilitating the identification and characterization of neoantigens through high-performance liquid chromatography coupled to tandem Mass Spectrometry.

Specifications

• Antigen: MHC Class I (H-2Db)
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: PBS with no preservative; pH 7.2
• Gene Accession No.: P01895, P01896, P01897, P01899, P01900, P14426, P14427
• Gene Symbols: H2-D1
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 14964
• Content And Storage: 4° C
• Form: Liquid

• Applications: Control, Flow Cytometry, Functional Assay
• Clone: 28-14-8
• Conjugate: Functional Grade
• Gene: H2-D1
• Gene Alias: H-2 class I histocompatibility antigen, D-B alpha chain; H-2D; H2-D; H-2D cell surface glycoprotein; H-2D(B); H2D1; H2-D1; H2DB; H2-DC1-beta transplantation antigen protein; H2L; H2LD; histocompatibility 2, D region locus 1; MHC class I antigen; MHC class I H2 antigen; MHC class I H-2Dp; MHC H2-D-q alpha-chain; Q5k protein
• Host Species: Mouse
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Mouse
• Product Type: Antibody
• Isotype: IgG2a κ

Safety and Handling

WARNING: Cancer and Reproductive Harm - www.P65Warnings.ca.gov

For Research Use Only.