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Invitrogen™ MHC Class I (H-2Kb) Monoclonal Antibody (AF6-88.5.5.3), Alexa Fluor™ 700, eBioscience™, Invitrogen™
Description
Description: This AF6-88.5.5.3 monoclonal antibody reacts with the H-2Kb MHC class I alloantigen. H-2Kb is involved in antigen presentation to T cells expressing CD3/TCR and CD8. Reactivity to other haplotypes (e.g. d, f, j, k, p, q, r, s, u, and v) has not been observed. Applications Reported: This AF6-88.5.5.3 antibody has been reported for use in flow cytometric analysis. Applications Tested: This AF6-88.5.5.3 antibody has been tested by flow cytometric analysis of mouse splenocytes. This may be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Alexa Fluor 700 emits at 723 nm and can be excited with the red laser (633-647 nm). Most instruments will require a 685 LP mirror and 710/20 band pass filter. Please make sure that your instrument is capable of detecting this fluorochrome. Excitation: 633-647 nm; Emission: 723 nm; Laser: Red Laser
Specifications
Specifications
| Antigen | MHC Class I (H-2Kb) |
| Applications | Flow Cytometry |
| Classification | Monoclonal |
| Clone | AF6-88.5.5.3 |
| Concentration | 0.2 mg/mL |
| Conjugate | Alexa Fluor 700 |
| Formulation | PBS with 0.09% sodium azide; pH 7.2 |
| Gene | H2-K1 |
| Gene Accession No. | P01901, P03991, P04223, P14428 |
| Gene Alias | class I major histocompatibility antigen H-2Kd; H-2 class I histocompatibility antigen, K-B alpha chain; H-2 class I histocompatibility antigen, K-D alpha chain; H-2 class I histocompatibility antigen, K-K alpha chain; H-2 class I histocompatibility antigen, K-Q alpha chain; H-2 class I histocompatibility antigen, K-W28 alpha chain; H-2K; H2-K; H-2K(B); H-2K(d); H-2K(K); H-2K(Q); H2-K1; histocompatibility 2, K1, K region; K-f; MHC class I antigen; MHC class I H2-K-b-alpha-2 cell surface glycoprotein; MHC class I heavy chain H2-K; MHC H2-K transplantation antigen |
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