Invitrogen™ MHC Class II (H2-Aa) Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA5116820

Catalog No. PIPA5116820

Description

Positive Samples: Mouse lung, Mouse kidney Immunogen sequence: IEADHVGTYG ISVYQSPGDI GQYTFEFDGD ELFYVDLDKK ETVWMLPEFG QLASFDPQGG LQNIAVVKHN LGVLTKRSNS TPATNEAPQA TVFPKSPVLL GQPNTLICFV DNIFPPVINI TWLRNSKSVA DGVYETSFFV NRDYSFHKLS YLTFIPSDDD IYDCKVEHWG LEEPVLKHWE PE.

MHC class II molecules are heterodimers of non-covalently associated alpha (31-34 kDa) and beta (26-29 kDa) chains. Major histocompatibility complex class II antigen presentation requires the participation of lysosomal proteases in two convergent processes. First, the antigens endocytosed by the antigen presenting cells must be broken down into antigenic peptides. Second, class II molecules are synthesized with their peptide-binding site blocked by invariant chain (Ii), and they aquire the capacity to bind antigens only after Ii has been degraded in the compartments where peptides reside. MHC class II molecules present exogenously derived antigen to CD4+ T lymphocytes, which are usually T helper cells. CD4 interacts with non-polymorphic residues of MHC class II. HLA and MHC antibodies play a significant role in Immunopeptidomics, facilitating the identification and characterization of neoantigens through high-performance liquid chromatography coupled to tandem Mass Spectrometry.

Specifications

• Antigen: MHC Class II (H2-Aa)
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: H2-Aa
• Gene Alias: A alpha; Aalpha; H-2 class II histocompatibility antigen, A-B alpha chain; H-2 class II histocompatibility antigen, A-F alpha chain; H-2 class II histocompatibility antigen, A-K alpha chain; H-2 class II histocompatibility antigen, A-Q alpha chain; H-2 class II histocompatibility antigen, A-R alpha chain; H-2 class II histocompatibility antigen, A-S alpha chain; H-2 class II histocompatibility antigen, A-U alpha chain; H2Aa; H-2Aa; H2-Aa; histocompatibility 2, class II antigen A, alpha; Ia1; Ia-1; IAalpha; I-Aalpha; major histocompatibility protein class II alpha chain; MHC class II antigen A alpha isoform-004; MHC-II I-A(d) alpha
• Host Species: Rabbit
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 14960
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid

• Applications: ELISA, Western Blot
• Concentration: 1.99 mg/mL
• Formulation: PBS with 50% glycerol and 0.09% sodium azide; pH 7.3
• Gene Accession No.: P01910, P04227, P04228, P14434, P14435, P14436, P14437, P14438, P23150
• Gene Symbols: H2-Aa
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 27-208 of mouse H2-Aa (NP_0345082).
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse
• Product Type: Antibody
• Isotype: IgG