MHC class II (I-A/I-E) Antibody (M5/114.15.2), Alexa Fluor™ 532, Novus Biologicals™

Rat Monoclonal Antibody

Supplier:  Novus Biologicals NBP143312AF532

Catalog No. NP143312A32

Description

MHC class II (I-A/I-E) Monoclonal specifically detects MHC class II (I-A/I-E) in Human, Mouse samples. It is validated for Western Blot, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen.

Specifications

• Antigen: MHC class II (I-A/I-E)
• Classification: Monoclonal
• Conjugate: Alexa Fluor 532
• Formulation: 50 mM sodium borate with 0.05% sodium azide
• Gene Symbols: H2-Ea-ps
• Immunogen: The immunogen for this antibody was MHC Class II.
• Primary or Secondary: Primary
• Test Specificity: The monoclonal antibody reacts with the mouse major histocompatibility complex class II, both I-A and I-E subregion-encoded glycoproteins (I-A b, I-A d, I-A q, I-E d, I-E k, not I-A f, I-A k, or I-A s). It detects a polymorphic determinant present on B cells, monocytes, macrophages, dendritic cells, and activated T lymphocytes from mice carrying the H-2 b, H-2 d, H-2 q, H-2 p, H-2 r and H-2 u but not from mice carrying the H-2 s or H-2 f haplotypes. The M5/114 mAb is reported to inhibit I-A-restricted T cell responses of the H-2 b, H-2 d, H-2 q, H-2 u but not H-2 f, H-2 k, or H-2 s haplotypes.
• Content And Storage: Store at 4°C in the dark.

• Applications: Western Blot, Flow Cytometry, Immunohistochemistry, Immunohistochemistry (Paraffin), Immunohistochemistry (Frozen)
• Clone: M5/114.15.2
• Dilution: Western Blot, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen
• Gene Alias: class II histocompatibility antigen, M alpha chain, D6S222E, DMA, HLA class II histocompatibility antigen, DM alpha chain, major histocompatibility complex, class II, DM alpha, MHC class II antigen DMA, Really interesting new gene 6 protein, RING6HLADM
• Host Species: Rat
• Quantity: 0.1 mL
• Gene ID (Entrez): 100504404
• Target Species: Human, Mouse
• Isotype: IgG2b κ