MHC class II (I-A/I-E) Antibody (M5/114.15.2) - BSA Free, Novus Biologicals™

Rat Monoclonal Antibody has been used in 11 publications

Catalog Number	Mfr. No.	Quantity
NBP14331205	Novus Biologicals NBP1433120.05MG	0.05 mg
NBP14331201	Novus Biologicals NBP1433120.1MG	0.1 mg
NBP1433125	Novus Biologicals NBP1433120.5MG	0.5 mg

Description

MHC class II (I-A/I-E) Monoclonal specifically detects MHC class II (I-A/I-E) in Human, Mouse samples. It is validated for Western Blot, Flow Cytometry, Immunohistochemistry, Immunoprecipitation, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen.

Specifications

• Antigen: MHC Class II
• Concentration: 0.5 mg/mL
• Applications: Western Blot, Flow Cytometry, Immunohistochemistry, Immunoprecipitation, Immunohistochemistry (Paraffin), Immunohistochemistry (Frozen)
• Conjugate: Unconjugated
• Regulatory Status: RUO
• Target Species: Mouse
• Gene Alias: class II histocompatibility antigen, M alpha chain, D6S222E, DMA, HLA class II histocompatibility antigen, DM alpha chain, major histocompatibility complex, class II, DM alpha, MHC class II antigen DMA, Really interesting new gene 6 protein, RING6HLADM
• Gene Symbols: HLA-DMA
• Isotype: IgG2b κ
• Purification Method: Affinity Purified
• Test Specificity: The monoclonal antibody reacts with the mouse major histocompatibility complex class II, both I-A and I-E subregion-encoded glycoproteins (I-A b, I-A d, I-A q, I-E d, I-E k, not I-A f, I-A k, or I-A s). It detects a polymorphic determinant present on B cells, monocytes, macrophages, dendritic cells, and activated T lymphocytes from mice carrying the H-2 b, H-2 d, H-2 q, H-2 p, H-2 r and H-2 u but not from mice carrying the H-2 s or H-2 f haplotypes. The M5/114 mAb is reported to inhibit I-A-restricted T cell responses of the H-2 b, H-2 d, H-2 q, H-2 u but not H-2 f, H-2 k, or H-2 s haplotypes.

• Clone: M5/114.15.2
• Dilution: Western Blot 1:100-1:2000, Flow Cytometry < 0.125 ug/10^6 cells, Immunohistochemistry 1:10-1:500, Immunoprecipitation 1:10-1:500, Immunohistochemistry-Paraffin 1:10-1:500, Immunohistochemistry-Frozen 1:10-1:500
• Classification: Monoclonal
• Host Species: Rat
• Research Discipline: Adaptive Immunity, Asthma, Cell Biology, Diabetes Research, Immunology, Major Histocompatibility Complexes
• Formulation: Aqueous, may contain carrier protein/stabilizer. with 0.09% Sodium Azide
• Gene ID (Entrez): 3108
• Immunogen: The immunogen for this antibody was MHC Class II.
• Primary or Secondary: Primary
• Content And Storage: Store at 4C. Do not freeze.

For Research Use Only