Invitrogen™ MHC Class II Monoclonal Antibody (YKIX334.2), FITC

Rat Monoclonal Antibody

Supplier:  Invitrogen™ MA181228

Catalog No. PIMA181228

Description

For FACS analysis, use 10 μL of the suggested working dilution to label 1x10^6 cells in 100 μL. Rat anti Dog MHC Class II Monomorphic antibody, clone YKIX334.2 recognizes a monomorphic epitope on canine MHC Class II and was classified at the First Canine Leucocyte Antigen Workshop (Cobbold et al. 1994).

MHC (major histocompatibility complex) class II molecules are transmembrane glycoproteins expressed on the surface of professional antigen-presenting cells, such as macrophages, dendritic cells and B cells. Before their exposition on the cell surface, the MHC class II molecules react with endocytosed exogenous antigens, which are then presented to the T cells. The antigen-binding grove between MHC class II alpha and beta chain is open at both ends and is 15-24 amino acid residues long. HLA and MHC antibodies play a significant role in Immunopeptidomics, facilitating the identification and characterization of neoantigens through high-performance liquid chromatography coupled to tandem Mass Spectrometry.

Specifications

• Antigen: MHC Class II
• Classification: Monoclonal
• Concentration: 0.1 mg/mL
• Formulation: PBS with 1% BSA and 0.09% sodium azide
• Host Species: Rat
• Purification Method: Protein G
• Regulatory Status: RUO
• Target Species: Canine
• Product Type: Antibody
• Isotype: IgG2a

• Applications: Flow Cytometry
• Clone: YKIX334.2
• Conjugate: FITC
• Gene Alias: beta 1 domain; B-L; B-L beta chain; B-L beta minor; B-LB; BLB major; BLB1; BLB2; B-LB2; B-LB21; B-LB21 major; BLB3; Bl-Beta II protein; B-LBII; class II histocompatibility antigen, B-L beta chain; class II histocompatibility antigen, M alpha chain; D6S222E; DMA; FECA-DRB; GSP-BLB1; GSP-BLB2; HLA class II histocompatibility antigen, DM alpha chain; HLADM; HLA-DMA; major histocompatibility class II antigen B-L beta; major histocompatibility complex class II B; Major histocompatibility complex class II beta chain BLB1; Major histocompatibility complex class II beta chain BLB1, (similar to HLA class II, D beta chain); major histocompatibility complex class II beta chain BLB1, (similar to HLA class II, D beta chain); Major histocompatibility complex class II beta chain BLB2, (similar to HLA class II, D beta chain); Major histocompatibility complex class II beta chain BLB3, (similar to HLA class II, D beta chain); major histocompatibility complex, class II, DM alpha; MHC 2; MHC Class II; MHC class II antigen; MHC class II antigen beta chain; MHC class II antigen B-F minor heavy chain; MHC class II antigen DMA; MHC class II antigen DRB; MHC Class II beta 1 and 2 domains; MHC class II beta 1 domain; MHC class II beta chain; MHC class II beta chain 1; MHC class II beta chain 2; MHC class II B-L beta chain; MHC II; MHCCII; MHCFECA-DRB; really interesting new gene 6 protein; RING6; S19; uncharacterized protein LOC101747454
• Immunogen: Con A activated canine peripheral blood cells.
• Quantity: 100 Tests
• Primary or Secondary: Primary
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Store in the dark.
• Form: Liquid