Invitrogen™ MHC I Polymorphic Monoclonal Antibody (ER-HR52)

Rat Monoclonal Antibody

Supplier:  Invitrogen™ MA181644

Catalog No. MA181644

Description

Clone ER-HR52 specifically recognizes mouse strains with the haplotypes H-2b, w16 and H-2d, p, q. Mouse strains with the haplotypes H-2f, r, s, w17, w23, w27 show weak reactivity with this antibody. For FACS analysis, use 10 μL of the suggested working dilution to label 1x10^6 cells in 100 μL.

The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In mice, this complex is referred to as the histocompatibility 2 (H-2) region. The polymorphic epitope is present on murine MHC class I molecules, which are expressed at varying levels on the majority of nucleated cells. HLA and MHC antibodies play a significant role in Immunopeptidomics, facilitating the identification and characterization of neoantigens through high-performance liquid chromatography coupled to tandem Mass Spectrometry.

Specifications

• Antigen: MHC I Polymorphic
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: PBS with 0.09% sodium azide; pH 7.4
• Gene Accession No.: P01895, P01896, P01897, P01899, P01900, P01901, P03991, P04223, P14426, P14427, P14428
• Gene Symbols: H2-Bl, H2-D1, H2-K1
• Immunogen: Adherent FI (CBAXBL) bone marrow stromal cells.
• Quantity: 250 μg
• Primary or Secondary: Primary
• Target Species: Mouse
• Product Type: Antibody
• Isotype: IgG2a

• Applications: Flow Cytometry, Immunohistochemistry (Frozen), Immunocytochemistry
• Clone: ER-HR52
• Conjugate: Unconjugated
• Gene: H2-D1
• Gene Alias: blastocyst MHC; class I major histocompatibility antigen H-2Kd; H2 blastocyst; H-2 class I histocompatibility antigen, D-B alpha chain; H-2 class I histocompatibility antigen, K-B alpha chain; H-2 class I histocompatibility antigen, K-D alpha chain; H-2 class I histocompatibility antigen, K-K alpha chain; H-2 class I histocompatibility antigen, K-Q alpha chain; H-2 class I histocompatibility antigen, K-W28 alpha chain; H2-B1; H2-Bl; H-2D; H2-D; H-2D cell surface glycoprotein; H-2D(B); H2-D1; H2-DC1-beta transplantation antigen protein; H-2K; H2-K; H-2K(B); H-2K(d); H-2K(K); H-2K(Q); H2-K1; histocompatibility 2, blastocyst; histocompatibility 2, D region locus 1; histocompatibility 2, K1, K region; K-f; MHC Class 1; MHC class I antigen; MHC class I antigen splice variant Bl.1; MHC class I H2 antigen; MHC class I H-2Dp; MHC class I H2-K-b-alpha-2 cell surface glycoprotein; MHC class I heavy chain H2-K; MHC H2-D-q alpha-chain; MHC H2-K transplantation antigen; Q5k protein
• Host Species: Rat
• Purification Method: Protein G
• Regulatory Status: RUO
• Gene ID (Entrez): 14963, 14964, 14972
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov