Invitrogen™ MIA3 Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA521389

Catalog No. PIPA521389

Description

Recommended positive controls: A549, HepG2. Predicted reactivity: Bovine (89%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Plays a role in the transport of cargos that are too large to fit into COPII-coated vesicles and require specific mechanisms to be incorporated into membrane-bound carriers and exported from the endoplasmic reticulum. This protein is required for collagen VII (COL7A1) secretion by loading COL7A1 into transport carriers. It may participate in cargo loading of COL7A1 at endoplasmic reticulum exit sites by binding to COPII coat subunits Sec23/24 and guiding SH3-bound COL7A1 into a growing carrier. Does not play a role in global protein secretion and is apparently specific to COL7A1 cargo loading. However, it may participate in secretion of other proteins in cells that do not secrete COL7A1. It is also specifically required for the secretion of lipoproteins by participating in their export from the endoplasmic reticulum (PubMed:27138255, PubMed:19269366). Required for correct assembly of COPII coat components at endoplasmic reticulum exit sites (ERES) and for the localization of SEC16A and membrane-bound ER-resident complexes consisting of MIA2 and PREB/SEC12 to ERES (PubMed:28442536). [UniProt].

Specifications

• Antigen: MIA3
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: MIA3
• Gene Alias: 9130229H14Rik; A930039G15Rik; ARNT; AU022327; B230399H06Rik; C219-reactive peptide; C80126; D320; Gm1525; Kiaa0268; melanoma inhibitory activity 3; melanoma inhibitory activity family member 3; melanoma inhibitory activity family, member 3; melanoma inhibitory activity protein 3; MIA family member 3, ER export factor; Mia3; RP11-378J18.4; Tango; TANGO1; transport and Golgi organization protein 1; Transport and Golgi organization protein 1 homolog; UNQ6077; UNQ6077/PRO20088
• Host Species: Rabbit
• Purification Method: Antigen Affinity Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 375056
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry
• Concentration: 0.3 mg/mL
• Formulation: 0.1M tris glycine with 20% glycerol and 0.01% thimerosal; pH 7
• Gene Accession No.: Q5JRA6
• Gene Symbols: MIA3
• Immunogen: Recombinant fragment corresponding to a region within amino acids 1 and 150 of MIA3 (Uniprot ID#Q5JRA6).
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG