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MIC A/B Mouse anti-Human, Brilliant Violet 605, Clone: 6D4, BD Optibuild™

Mouse Monoclonal Antibody

Manufacturer:  BD Biosciences 742324

Catalog No. BDB742324


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Description

Description

The 6D4 monoclonal antibody specifically binds to the human MHC class I polypeptide-related sequence A (MICA, aka /PERB11.1) and B (MICB. aka /PERB11.2) proteins. These ñ70 kDa transmembrane glycoproteins are homologs of the major histocompatibility complex class I molecules although they lackassociation with β2 microglobulin. The MHC class I-related MICA and MICB chains are expressed by some gut epithelial cells in vivo. MICA and MICB expression by other epithelial cells and cell types, including fibroblasts and endothelial cells, is induced by stress, eg, stress caused by bacterial and viral infections, autoimmunity or cellular transformation. Epithelial cell expression of MICA and MICB has also been detected in transplanted kidneys and pancreas that show histological signs of rejection and or cellular injury. This suggests their potential role in transplant immunopathology. MICA and MICB are ligands for NKG2D (CD314), an activating receptor expressed by natural killer (NK) cells,γδ T cells,CD8+ andsome CD4+ αβ T cells. The 6D4 antibody reportedly blocks NKG2D-positive NK cell- and T cell-mediated cytotoxicity against MICA/B-positive target cells.

This antibody is conjugated to BD Horizon™ BV605 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 407nm and Em Max of 602nm, BD Horizon BV605 can be excited by a violet laser and detected with a standard 610/20nm filter set. BD Horizon BV605 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an Em max at 605nm. Due to the excitation of the acceptor dye by the green (532 nm) and yellow-green (561 nm) lasers, there will be significant spillover into the PE and BD Horizon PE-CF594 detectors off the green or yellow-green lasers. BD Horizon BV605 conjugates are very bright, often exhibiting brightness equivalent to PE conjugates and can be used as a third color off of the violet laser. BV605 is a tandem fluorochrome that combines BD Horizon BV421 and Cy™3.5. These conjugates are very bright, exhibiting similar brightness to equivalent PE conjugates. Due to the excitation of Cy3.5 by the green (532nm) and yellow-green (561nm) lasers, there will be significant spillover of the BD Horizon BV605 signal into the PE and BD Horizon PE-CF594 detectors off the green or yellow-green lasers.
Specifications

Specifications

MIC A/B
0.2mg/mL
Aqueous buffered solution containing ≤0.09% sodium azide.
MHC class I polypeptide-related sequence A/B, PERB11.1/ PERB11.2
MICA transfected cells
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon BV605 under optimal conditions that minimize unconjugated dye and antibody.
RUO
Primary
Human
6D4
Brilliant Violet 605
Affinity Purified
Murine
Mouse IgG2a, κ
50μg
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Monoclonal
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