Invitrogen™ MICA/B Monoclonal Antibody (6D4), Functional Grade, eBioscience™

Mouse Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
50-145-70	Invitrogen™ 16578885	500 μg

Description

Description: The 6D4 monoclonal antibody reacts with the human major histocompatibility complex (MHC) class I chain-related (MIC), MICA and MICB proteins. MICA and MICB are related proteins of 83% amino acid similarity, and show homology with classical human leukocyte antigen (HLA) molecules. The structure of MICA and MICB are similar to classical HLA class I chains, however they do not bind beta2 microglobulin or bind peptide typical of HLA class I. MICA and MICB are expressed on the cell surface of endothelial cells, fibroblasts, gastric epithelium and PHA-stimulated T cells, and act as a ligand for NKGD2 expressed on the surface of NK cells, gammadelta T cells and alpha beta CD8+ T cells. There is evidence to suggest that human cytomegalovirus (HCMV) subverts NK cell detection by inhibiting the function of MICB. Furthermore, MICA and MICB expression has been detected in several epithelial tumours isolated from breast, lung, ovary, prostate, colon and kidney. Applications Reported: This 6D4 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunohistochemical staining. Applications Tested: This 6D4 antibody has been tested by flow cytometric analysis of HeLa cells. This can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is ...

MICA encodes the higly polymorphic MHC (HLA) class I chain-related gene A. The protein product is expressed on the cell surface, although unlike canonical class I molecules does not seem to associate with beta-2-microglobulin. It is thought that MICA functions as a stress-induced antigen that is broadly recognized by intestinal epithelial gamma delta T cells.

Specifications

• Antigen: MICA/B
• Concentration: 1 mg/mL
• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry, Immunoprecipitation
• Conjugate: Functional Grade
• Host Species: Mouse
• Regulatory Status: RUO
• Formulation: PBS with no preservative; pH 7.2
• Gene Accession No.: Q29980, Q29983
• Gene ID (Entrez): 100507436, 4277
• Primary or Secondary: Primary
• Purification Method: Affinity Chromatography

• Clone: 6D4
• Content And Storage: 4°C
• Classification: Monoclonal
• Form: Liquid
• Isotype: IgG2a κ
• Target Species: Human
• Gene: MICA
• Gene Alias: HLA class I antigen; MHC class I chain-related protein A; MHC class I chain-related protein B; MHC class I mic-B antigen; MHC class I polypeptide-related sequence A; MHC class I polypeptide-related sequence B; MHC class I related sequence A; MHC class I-like molecule PERB11.2-IMX; MICA; MIC-A; MICB; MIC-B; PERB11.1; PERB11.2; sMICA; sMICB; soluble MICA; soluble MICB; stress inducible class I homolog; truncated MHC class I polypeptide-related sequence A
• Gene Symbols: MICA, MICB
• Product Type: Antibody