Invitrogen™ MID1 Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA536305

Catalog No. PIPA536305

Description

This antibody detects endogenous protein at a molecular weight of 75 kDa. Purity is >95% by SDS-PAGE.

Midline-1 (Tripartite motif-containing protein 18, Putative transcription factor XPRF, RING finger protein 59) is a 667 amino acid protein encoded by the human gene MID1. Midline-1 belongs to the TRIM/RBCC family and contains two B box-type zinc fingers, one B30.2/SPRY domain, one COS domain, one fibronectin type-III domain and one RING-type zinc finger. Midline-1 is believed to have E3 ubiquitin ligase activity which targets the catalytic subunit of protein phosphatase 2 for degradation. It is a cytoplasmic protein found as a homodimer or heterodimer with Midline-2. It also interacts with IGBP1 (Lymphocyte signaling protein A4). Defects in MID1 are the cause of Opitz syndrome type I (OS-I). OS-I is an X-linked recessive disorder characterized by hypertelorism, genital-urinary defects such as hypospadias in males and splayed labia in females, lip-palate-laryngotracheal clefts, imperforate anus, developmental delay and congenital heart defects.

Specifications

• Antigen: MID1
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: MID1
• Gene Alias: 61B3-R; BBBG1; DXHXS1141; E3 ubiquitin-protein ligase Midline-1; Finger on X and Y (in rat only on X); Fxy; GBBB1; Mid1; Midin; midline 1; midline 1 (Opitz/BBB syndrome); midline 1 RING finger protein; Midline1; midline-1; OGS1; Opitz/BBB syndrome; OS; OSX; Putative transcription factor XPRF; RING finger protein 59; RING finger protein Midline-1; RING-type E3 ubiquitin transferase Midline-1; RNF59; TRIM18; tripartite motif protein TRIM18; tripartite motif-containing protein 18; XPRF; zinc finger on X and Y, mouse, homolog of; ZNFXY
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 17318, 4281, 54252
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Immunohistochemistry (Paraffin), Western Blot
• Concentration: 1 mg/mL
• Formulation: PBS with 50% glycerol and 0.02% sodium azide; pH 7.2
• Gene Accession No.: O15344, O70583, P82458
• Gene Symbols: MID1
• Immunogen: Synthetic peptide corresponding to amino acids 71-120 of human Midline-1.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG