Midkine Polyclonal Antibody, Biotin, PeproTech®, Invitrogen™

Rabbit Polyclonal Antibody

Catalog Number	Mfr. No.	Quantity
50-271-8643	Invitrogen™ 500P171BT500UG	500 μg

Description

AA Sequence of recombinant protein: VAKKKDKVKK GGPGSECAEW AWGPCTPSSK DCGVGFREGT CGAQTQRIRC RVPCNWKKEF GADCKYKFEN WGACDGGTGT KVRQGTLKKA RYNAQCQETI RVTKPCTPKT KAKAKAKKGK GKD. Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human Midkine. Anti-Human Midkine-specific antibody was purified by affinity chromatography and then biotinylated. Sandwich ELISA: To detect hMidkine by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human Midkine (500-P171) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant hMidkine. Western Blot: To detect hMidkine by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hMidkine is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. 500-P171BT-1MG will be provided as 2 x 500 μg

Midkine is a retinoic acid-responsive, heparin-binding growth factor expressed in various cell types during embryogenesis. It promotes angiogenesis, cell growth, and cell migration. Midkine is also expressed in several carcinomas, suggesting that it may play a role in tumorigenesis, perhaps through its effects on angiogenesis.

Specifications

• Antigen: Midkine
• Applications: ELISA, Western Blot
• Conjugate: Biotin
• Host Species: Rabbit
• Target Species: Human
• Gene Accession No.: P21741
• Gene ID (Entrez): 4192
• Immunogen: E.coli-derived Recombinant Human Midkine.
• Purification Method: Antigen affinity chromatography
• Gene: Mdk

• Concentration: 0.1-1.0 mg/mL
• Classification: Polyclonal
• Form: Lyophilized
• Regulatory Status: RUO
• Formulation: PBS with no preservative
• Gene Alias: amphiregulin-associated protein; ARAP; FLJ27379; MDK; Mek; Midgestation and kidney protein; midkine; midkine (neurite growth-promoting factor 2); MK; MK1; NEGF2; Neurite outgrowth-promoting factor 2; Neurite outgrowth-promoting protein; Retanoic acid-responsive protein; retinoic acid inducible factor; retinoic acid-induced differentiation factor; retinoic acid-responsive protein
• Gene Symbols: Mdk
• Primary or Secondary: Primary
• Content And Storage: -20°C