Invitrogen™ MIDN Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA550521

Catalog No. PA550521

Description

The antibody detects endogenous levels of total MIDN protein.

Expression of the midnolin gene is developmentally regulated: it is strongly expressed at the mesencephalon (midbrain) of the embryo in day 12.5 (E12.5) mice. The midnolin encodes a protein of 508 amino acids (aa), which contains a Ubiquitin-like domain. The intracellular distribution of the midnolin was studied by using midnolin-green fluorescent protein (GFP) fusion proteins. Midnolin was found to be localized in the nucleus and nucleolus, but not in the cytoplasm. The nucleolar localization signal was determined to be a 28aa peptide located at the C-terminal region of the midnolin. May be involved in regulation of genes related to neurogenesis in the nucleolus.

Specifications

• Antigen: MIDN
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: MIDN
• Gene Alias: 3000003C15Rik; DKFZp547M072; midbrain nucleolar protein; Midn; Midnolin
• Host Species: Rabbit
• Purification Method: Protein A
• Regulatory Status: RUO
• Gene ID (Entrez): 314623, 59090, 90007
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Immunohistochemistry, Western Blot, Immunocytochemistry
• Concentration: 1 mg/mL
• Formulation: TBS with 50% glycerol and 0.02% ProClin 300; pH 7.4
• Gene Accession No.: D4AE48, Q3TPJ7, Q504T8
• Gene Symbols: MIDN
• Immunogen: Synthetic peptide corresponding to residues near the N terminal of human Midnolin.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG