MITF Antibody (SPM290), Novus Biologicals™

Catalog No.	Quantity
NBP24516000	0.02 mg
NBP24516001	0.1 mg
NBP24516002	0.2 mg

Catalog No. NBP24516000 Supplier Novus Biologicals Supplier No. NBP2451600.02MG

Description

Mouse Monoclonal Antibody

Ensure accurate, reproducible results in Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence

MITF Monoclonal specifically detects MITF in Human, Mouse (Negative), Rat (Negative) samples. It is validated for Immunohistochemistry, Immunohistochemistry-Paraffin.

Specifications

• Antigen: MITF
• Applications: Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence
• Classification: Monoclonal
• Clone: SPM290
• Concentration: 0.2mg/mL
• Conjugate: Unconjugated
• Dilution: Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml, Immunofluorescence 0.5 - 1.0 ug/ml
• Formulation: 10mM PBS and 0.05% BSA with 0.05% Sodium Azide
• Gene Alias: BHLHE32, bHLHe32MI, Class E basic helix-loop-helix protein 32, microphthalmia-associated transcription factor, Waardenburg syndrome, type 2A, WS2A
• Gene Symbols: MITF
• Host Species: Mouse
• Immunogen: NH2 terminus fragment of human Mi protein
• Purification Method: Protein A or G purified
• Quantity: 0.02 mg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 4286
• Test Specificity: MITF (microphthalmia transcription factor) is a basic helix-loop-helix-leucine-zipper (bHLH-Zip) transcription factor that regulates the development and survival of melanocytes and retinal pigment epithelium, and also is involved in transcription of pigmentation enzyme genes such as tyrosinase TRP1 and TRP2. MITF has been shown to be phosphorylated by MAP kinase in response to c-kit activation, resulting in upregulation of MITF transcriptional activity. Mutations of the MITF gene are associated with the autosomal dominant hereditary deafness and pigmentation condition, Waardenburg Syndrome type 2A. Multiple isoforms of MITF exist, including MITF-A, MITF-B, MITF-C, MITF-H, and MITF-M, which differ in the amino-terminal domain and in their expression patterns. The MITF-M isoform is restricted to the melanocyte cell lineage. Anti-MITF, D5, recognizes a nuclear protein, which is expressed in the majority of primary and metastatic epithelioid malignant melanomas as well as in normal melanocytes, benign nevi and dysplastic nevi.
• Target Species: Human, Mouse (Negative), Rat (Negative)
• Content And Storage: Store at 4C.
• Form: Purified
• Isotype: IgG1 κ

For Research Use Only